Uses
N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) is a granzyme B substrate that allows accurate measurement of granzyme B activity[1].
Biological Activity
Primary Target
Colorimetric substrate for granzyme B', 'kcat/Km = 6.6 x 104 M-¹ sec-¹ as substrate for granzyme B
References
[1] Kristina Fritsch, et al. Suppression of granzyme B activity and caspase-3 activation in leukaemia cells constitutively expressing the protease inhibitor 9. Ann Hematol. 2013 Dec;92(12):1603-9. DOI:
10.1007/s00277-013-1846-6