Description
Fatty acid amide hydrolase (FAAH) is the enzyme responsible for hydrolysis and inactivation of fatty acid amides including anandamide and oleamide. PF-
750 is a potent, time-
dependent, irreversible FAAH inhibitor with IC
50 values of 0.6 and 0.016 μM when preincubated with recombinant human FAAH for 5 and 60 minutes, respectively. Activity-
based profiling of various human and murine tissue proteome samples revealed that PF-
750 is highly selective for FAAH relative to other serine hydrolases, showing no discernable off-
site activity up to 500 μM.
Uses
Fatty acid amide hydrolase (FAAH) inhibitor, selectively inhibiting FAAH within the central nervous system
Definition
ChEBI: N-phenyl-4-(3-quinolinylmethyl)-1-piperidinecarboxamide is a member of quinolines.
Biochem/physiol Actions
PF-750 is a potent, time-dependent, irreversible FAAH inhibitor with IC50 values 0.6 and 0.016 μM when preincubated with recombinant human FAAH for 5 and 60 minutes, respectively. Fatty acid amide hydrolase (FAAH) is the enzyme responsible for hydrolysis and inactivation of fatty acid amides including anandamide and oleamide. Activity-based profiling of various human and murine tissue proteome samples revealed that PF-750 is highly selective for FAAH relative to other serine hydrolases, showing no discernable off-site activity up to 500 μM. PF-750 shows 10-fold better potency than URB597 (Sigma# U4133) after 30 min preincubation. PF-750 is highly selective on FAAH. Even at as high as 500 μM, it had no interactions with many tested enzymes, but URB597 and other known FAAH inhibitors did not perform well at low concentraction (100 μM).
References
[1] KYUNGHYE AHN. Novel Mechanistic Class of Fatty Acid Amide Hydrolase Inhibitors with Remarkable Selectivity[J]. Biochemistry Biochemistry, 2007, 46 45: 13019-13030. DOI:
10.1021/bi701378g
