Chemical Properties
greenish blue powder
Uses
Chicago Sky Blue 6B is a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry. It has also been used as a filling in glass pipette electrodes for electrophysiological recordings in the brain sections of rats.
Uses
Competitive glutamate uptake inhibitor
Preparation
3,3′-Dimethoxylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-1,3-disulfonic acid (2 Moore) coupled.
General Description
Bright greenish-blue solid or dark blue powder.
Air & Water Reactions
Azo dyes can be explosive when suspended in air at specific concentrations. Slightly soluble in water.
Reactivity Profile
CHICAGO SKY BLUE 6B is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Cleavage of the diazo group by reducing agents may lead to benzidine production.
Fire Hazard
Flash point data for CHICAGO SKY BLUE 6B are not available; however, CHICAGO SKY BLUE 6B is probably combustible.
Biological Activity
Potent inhibitor of L-glutamate uptake into synaptic vesicles.
Biochem/physiol Actions
Chicago Sky Blue 6B is a large organic acid, structurally related to glutamate, that is a potent and efficient competitive inhibitor of vesicular glutamate uptake.
Safety Profile
An experimental
teratogen. Other experimental reproductive
effects. Mutation data reported. When
heated to decomposition it emits very toxic
fumes of NOx Na2O, and SOx
Properties and Applications
colourful green light blue. Blue powder. Soluble in water solution for bright blue, solubility of 50 g/L (80 ℃), slightly soluble in soluble fiber element, insoluble in other organic solvents. The strong sulfuric acid solution for blue-ray green, blue for diluted; In nitric acid solution for red light purple. The dye solution to join 10% no change sulfuric acid; Add thick sodium hydroxide solution for red light blue. Cellulose fiber of dyeing, the dye well after sex is very good, in 60 ~ 80 ℃ when supreme affinity, discharge sex is very good. In dyeing of copper (colored light turned green, a slight change in iron ion. Mainly used for cotton, viscose and other cellulose fibers, silk, nylon, rayon/nylon blend fabric, paper and biological stain can also be used for cotton and viscose fabric is mainly used for direct printing of cotton, rayon and other cellulose fiber, silk, nylon, rayon / nylon blend fabric, paper and biological stain can also be used for cotton and viscose fabric direct printing.
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Standard
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Acid Resistance
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Alkali Resistance
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Light Fastness
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Soaping
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Water
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Fading
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Stain
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Fading
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Stain
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ISO
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5
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4
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1
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2
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2
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AATCC
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5
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1
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1
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3-4
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1
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Purification Methods
Free it from other electrolytes by adding aqueous sodium acetate to a boiling solution of the dye in distilled water. After standing, the salted-out dye is filtered on a Büchner funnel, the process being repeated several times. Finally, the precipitated dye is boiled several times with absolute EtOH to wash out any sodium acetate, then dried (as the sodium salt) at 105o. [McGregor et al. Trans Faraday Soc 58 1045 1962, Beilstein 16 II 259.]
