Chemical Properties
Sulforhodamine G is an orange-red solid soluble in water. It is a fluorescent compound with an excitation peak at 531nm and an emission peak at 552nm.
Uses
Sulfordamine G is a useful research chemical compound used as fluorescent substrates for flow cytometric evaluation of efflux inhibition in ABCB1, ABCC1, and ABCG2 transporters.
Uses
Sulforhodamine G can be incorporated in composites as a dye for characterizing fluorescent correlation spectroscopy based images. It can also be used in the development of an acceptor molecule that facilitates the emission of white light in a light emitting hybrid.
Preparation
4-Formylbenzene-1,3-disulfonic acid and 3-(Ethylamino)-4-methylphenol?condensation, the products with sulfuric acid dehydration, again oxidation, and finally into sodium salt.
Definition
ChEBI: Sulforhodamine G is an organic sodium salt and a xanthene dye. It has a role as a fluorochrome. It contains a sulforhodamine G anion.
General Description
Sulforhodamine G is an ambipolar fluorescent dye that is used as an acceptor dye for organic electronics. It is a water soluble and hydrophilic dye that shows an absorption peak at a wavelength of 529 nm.
Staining Procedures
The specimen is treated for 5 seconds in a 1 mM freshly prepared and filtered solution of sulforhodamine G before sectioning. Hand sections are mounted in paraffin oil for fluorescence microscopy. The distribution of induced fluorescence corresponds to the water content of the tissue. For details, see Watt et al. (1996).
Properties and Applications
bright yellow peach.
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Standard
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Light Fastness
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Soaping
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Persperation Fastness
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Oxygen bleaching
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Fastness to seawater
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Fading
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Stain
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Fading
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Stain
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Fading
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Stain
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ISO
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3
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2
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3
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3
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1-2
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2-3
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