Uses
The most commonly used reagents for determining acid phosphatase are phenolphthalein diphosphate or its tetrasodium salt, thymolphthalein disodium monophosphate, and naphthylamine disodium monophosphate. These substances are classic phosphate derivatives used to determine pH values and are usually used in sodium salt form. Among these reagents, phenolphthalein diphosphate or its tetrasodium salt is more effective, easier to observe, less expensive than other reagents, and has low toxicity of the phenolphthalein released into the medium.
Chemical Properties
SLIGHTLY YELLOW FINE CRYSTALLINE POWDER
Enzyme inhibitor
This water-soluble reagent (FW = 491.26 g/mol), also known as phenolphthalein diphosphate and phenolphthalein phosphate, is an alternative substrate for phosphomonoesterases (e.g., alkaline phosphatase), as is the monophosphate. Hydrolysis of the phosphate group generates free phenolphthalein which is red in alkaline conditions. Target(s): inositol 1,4,5-trisphosphate receptors, weakly inhibited; phosphoprotein phosphatase.
