Uses
DRAQ 5 is a stain for live or fixed cells and is used to visualize DNA. It exhibits excitation and emission at 647 and 681nm, respectively. DRAQ 5 is suitable for multiplex detection applications with other fluorophores.
Background
DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications.
References
[1] Smith, P.J. et al. (2000) Cytometry 40, 280-91.
[2] Wiltshire, M. et al. (2000) Cytometry 39, 217-23.
[3] Smith, P.J. et al. (1999) J Immunol Methods 229, 131-9.
