Uses
Myelin Basic Protein (MHP4-14), a synthetic peptide comprising residues 4-14 of myelin basic protein, is a very selective PKC substrate (Km=7 μM). Myelin Basic Protein is not phosphorylated by cyclic AMP-dependent protein kinase, casein kinases I and II, Ca2+/calmodulin-dependent protein kinase II, or phosphorylase kinase, and can be routinely used for the assay of protein kinase C with low background in the crude tissue extracts[1][2].
References
[1] Farrar YJ, et al. A phosphatase resistant substrate for the assay of protein kinase C in crude tissue extracts. Biochem Biophys Res Commun. 1991;180(2):694-701. DOI:
10.1016/s0006-291x(05)81121-0[2] Yasuda I, et al. A synthetic peptide substrate for selective assay of protein kinase C. Biochem Biophys Res Commun. 1990;166(3):1220-1227. DOI:
10.1016/0006-291x(90)90996-z
