D-FRUCTOSE DEHYDROGENASE

Uses

D-Fructose Dehydrogenase from Gluconobacter sp has been used as a standard in the carbohydrate binding specificity assay and SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis).

Biochem/physiol Actions

D-fructose dehydrogenase is a heterotrimeric membrane-bound enzyme commonly seen in various Gluconobacter sp. especially in Gluconobacter japonicus (Gluconobacter industrius). It has a molecular mass of ca. 140 kDa, consisting of subunits I (67kDa), II (51 kDa), and III (20 kDa) and catalyzes the oxidation of D-fructose to produce 5-keto-D-fructose. The enzyme is a flavoprotein-cytochrome c complex with subunits I and II covalently bound to flavin adenine dinucleotide (FAD) and heme C as prosthetic groups, respectively.