Description
Pyocyanine (85-66-5), a redox-active phenazine produced by?P. aeruginosa?and other pathogens, is an electron receptor, which stimulates redox cycling in bacteria as well as in a variety of human cell lines. It enhances oxidative metabolism, which increases the formation of intracellular reactive oxygen species (ROS) via reduction of NADPH. Pyocyanine accelerates neutrophil apoptosis?in vitro. Pyocyanine production by?P. aeruginosa?suppresses the acute inflammatory response by pathogen-driven acceleration of neutrophil apoptosis and associated reduction of local inflammation.
Uses
Pyocyanin is an antibiotic accelerator of neutrophil apoptosis.
Uses
Pyocyanin has been used:
- To disable the ability of forkhead box A2 (FOXA2) to regulate goblet cell hyperplasia and metaplasia (GCHM) and mucin expression.
- To activate nuclear factor (erythroid-derived 2)-like 2 (NRF2) through the reactive oxygen species (ROS)-inducible epidermal growth factor receptor (EGFR)-phosphoinositide 3-kinase (PI3K) cellular signal transduction pathway and its downstream effectors.
- To stimulate Pseudomonas aeruginosa PAO1 cell line adhesion and invasion in human lung carcinoma A549 cells via reactive oxygen species (ROS) production.
General Description
Pyocyanin is a secondary metabolite, which is produced by
Pseudomonas aeruginosa.
Biochem/physiol Actions
Pyocyanin, a blue-green pigment belonging to phenazine pigments, is a redox-active phenazine. Pyocyanin is an electron receptor, which stimulates redox cycling in bacteria, liver cells, and human epithlial cell lines. Pyocyanin enhances the oxidative metabolism, which in turn increases the formation of intracellular reactive oxygen species (ROS) via reduction of NADPH. Pyocyanin also increases the release of IL-8 by airway epithelial cells both in vitro and in vivo. This involves signal transduction pathways that include oxidants, protein tyrosine kinases, and MAP kinases. IL-8 secretion by these cells is in synergy with inflammatory cytokines. Pyocyanin has been shown to accelerate neutrophil apoptosis in vitro, resulting in resolution of acute inflammation, which is beneficial for bacteria survival.
Enzyme inhibitor
This natural pigment (FW = 210.24 g/mol), which has an antifungal activity, is a secretory agent produced by Pseudomonas aeruginosa (formerly P. pyocyanea and Bacillus pyocyaneus). It is a blue solid that is soluble in chloroform and pyridine generates reactive oxygen species, such as superoxide and hydrogen peroxide. Pyocyanine is slightly soluble in cold water and the aqueous blue solution can be decolorized under alkaline conditions upon the addition of glucose or sodium hydrosulfite. Target(s): catalase; cytochrome P450 leukotriene B4 w-oxidation; and diamine oxidase.
Purification Methods
It crystallises from H2O as dark blue needles. The picrate has m 190o (dec). [Beilstein 23 H 395, 23 I 59, 23 II 234, 23/8 V 395.]
References
1) Rada and Leto (2013),?Pyocyanin effects on respiratory epithelium: relevance in Pseudomonas Aeruginosa airway infections; Trends Microbiol.,?21?73
2) Reszka?et al. (2012),?Inactivation of the potent Pseudomonas aeruginosa cytotoxin pyocyanin by airway peroxidases and nitrite; Am. J. Physiol. Lung Cell Mol. Physiol.,?302?L1044
