1H-Indole-3-acetic acid, 5-phenyl-

1H-Indole-3-acetic acid, 5-phenyl- Chemische Eigenschaften,Einsatz,Produktion Methoden

• Biologische Aktivität

  5-Ph-IAA is a derivative of IAA. 5-Ph-IAA is a ligand that together with the OsTIR1 (F74G) mutant establishes the auxin-inducible degron 2 system (AID2). AID2 induces rapid and efficient depletion of mAID fusion proteins to study protein function in living cells. AID2 can suppress tumors.

• in vitro

  HCT116 cells constitutively expressing OsTIR1(F74G) are exposed to 5-Ph-IAA (1 μM; 6 hours), leading to DHC1-mAC degradation, which means the AID2 system better than the original AID system in generating conditional mutant cell lines.

  Western Blot Analysis

  Cell Line:	HCT116 cells
  Concentration:	1 μM
  Incubation Time:	6 hours
  Result:	Depleted DHC1-mAC efficiently in HCT116 cells constitutively expressing OsTIR1(F74G).

• in vivo

  5-Ph-IAA (0-10 mg/kg; intraperitoneally injection; every day; 7 days), used for the AID2 system , is sufficient to deplete mAID-BRD4 and TOP2A-mAC, leading to tumor suppression.

  Animal Model:	Balb/c-nu female mice (7 weeks old; 16–20 g ) bearing mAID-BRD4 and TOP2A-mAC xenograft tumours cells
  Dosage:	0 mg/kg, 1 mg/kg , 3 mg/kg and 10 mg/kg
  Administration:	Intraperitoneally injection; every day; 7 days
  Result:	Displayed significant tumor suppression of mAID-BRD4 xenografts and TOP2A-mAC xenografts .

• Lager

  Store at -20°C

1H-Indole-3-acetic acid, 5-phenyl- Upstream-Materialien And Downstream Produkte

1H-Indole-3-acetic acid, 5-phenyl- Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global(28)Suppliers