K145 (0-10 μM; 24-72 hours; U937 cells) treatment significantly inhibits the growth of U937 cells in a concentration-dependent manner.
K145 (10 μM; 24 hours; U937 cells) ) treatment significantly induces apoptosis in U937 cells.
K145 (4-8 μM; 3 hours; U937 cells) treatment decreases the phosphorylation of ERK and Akt.
Treatment with K145 (10 μM) causes a decrease of total cellular S1P without significant effects on ceramide levels.
Cell Viability Assay
Cell Line: | < td class="col2"> U937 cells
Concentration: | 0 μM, 4 μM, 6 μM , 8 μM, 10 μM |
Incubation Time: | 24 hours, 48 hours, 72 hours | td>
Result: | Significantly inhibited the growth of U937 cells in a concentration-dependent manner. |
Apoptosis Analysis
Cell Line: | U937 cells |
Concentration: | 10 μM |
Incubation Time: | 24 hours |
Result: | Significantly induced apoptosis in U937 cells. |
Western Blot Analysis < /p>
Cell Line: | U937 cells |
Concentration: | 4 μM, 8 μM |
Incubation Time: < /td> | 3 hours |
Result: | Phosphorylated ERK and Akt wer e decreased. |