iCRT 14 Struktur

iCRT 14

Bezeichnung:iCRT 14

CAS-Nr677331-12-3

Englisch Name:iCRT 14

CBNumberCB92546789

SummenformelC21H17N3O2S

Molgewicht375.44

MOL-Datei677331-12-3.mol

iCRT 14 physikalisch-chemischer Eigenschaften

Schmelzpunkt	183-186°C
Siedepunkt	567.0±60.0 °C(Predicted)
Dichte	1.29±0.1 g/cm3(Predicted)
storage temp.	2-8°C
Löslichkeit	DMSO: ≥10mg/mL
Aggregatzustand	powder
pka	2.09±0.12(Predicted)
Farbe	yellow to orange
InChIKey	NCSHZXNGQYSKLR-UNOMPAQXSA-N

Sicherheit

Kennzeichnung gefährlicher	Xn
R-Sätze:	22
WGK Germany	3

Gefahreninformationscode (GHS)

iCRT 14 Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden
A potent inhibitor of β-catenin-responsive transcription (CRT) (IC50 = 40.3 nM in assays of Wnt pathway activity). Studies suggest that it may directly influence the interaction between β-catenin and TCF4. It induces marked G0/G1 cell cycle arrest in HCT-116 and HT29 cell lines.
Verwenden
iCRT14 may be used in Wnt /β-catenin-mediated cell signaling studies.
Biologische Aktivität
iCRT14 is a β-catenin/Tcf inhibitor with a Ki value of 54 ± 5.2 μM in the HFP assay.
Biochem/physiol Actions
iCRT14 belongs to the thiazolidinedione class of β-catenin-responsive transcription inhibitors. It decreases the levels of Dishevelled protein and modulates the binding of T-cell factor (TCF) to DNA. It results in consistent decrease in reduction of cell proliferation and tumor growth in colon cancer cells.
in vitro
iCRT14 inhibits the transcriptional activity of canonical Wnt signaling, downregulates Wnt/β-catenin-induced target genes, and inhibits the growth of colorectal cancer cells in vivo. iCRT14 moderately reduced Dvl without affecting Dvl phosphorylation. In mammalian HEK293 cells, iCRT14 inhibits the Wnt response element STF16-luc reporter gene with IC50 of 40.3 nM. In addition to affecting the TCF-β-catenin interaction, iCRT14 also interferes with the binding of TCF to DNA.
in vivo
In HCT116 and HT29 xenograft models, iCRT14 caused a significant decrease in CycD1 and decreased tumor proliferation. In addition, the initial tumor growth rate decreased significantly during the first three weeks of dosing. After 19 days of administration, the tumor growth rate returned to the control level. During the study, the mice did not show any symptoms of systemic toxic effects or weight loss. The modified compound can be rapidly metabolized in the body, and its bioavailability is low.
Lager
Store at +4°C
Einzelnachweise
[1] gonsalves f c, klein k, carson b b, et al. an rnai-based chemical genetic screen identifies three small-molecule inhibitors of the wnt/wingless signaling pathway. proceedings of the national academy of sciences, 2011, 108(15): 5954-5963.

iCRT 14 Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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