7-azido-4-methylcoumarin

7-azido-4-methylcoumarin Chemische Eigenschaften,Einsatz,Produktion Methoden

• Verwenden

  7-Azido-4-methylcoumarin (AzMC) is a fluorogenic probe useful for the detection of hydrogen sulfide (H₂S). The aromatic azide moiety is selectively reduced in the presence of H₂S, producing the fluorescent 7-amino-4-methylcoumarin (AMC) with a concomitant increase in fluorescence with λex = 365nm and λem = 450nm. The probe can be used both to monitor enzymatic production of H₂S in vitro and also to visualize H₂S in cells. The probe is highly sensitive and selective for H₂S, providing a linear detection range in vitro between 200nm – 100μM H₂S with 10μM probe, and reacting selectively with H₂S in the presence of mM concentrations of cysteine, homocysteine, glutathionine and many other common biological analytes.
  
  Caution: Use of this product with DTT, TCEP and/or biological thiols >25mM should be avoided for maximum efficiency.
  
  AzMC is readily soluble in DMSO.

7-azido-4-methylcoumarin Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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