Elastase (Schweine Pankreas)

Bezeichnung:Elastase (Schweine Pankreas)

CAS-Nr39445-21-1

Englisch Name:Elastase

CBNumberCB8394260

SummenformelNULL

Molgewicht0

MOL-DateiMol file

Synonyma

Elastase (Schweine Pankreas)

Elastase (Schweine Pankreas) physikalisch-chemischer Eigenschaften

RTECS-Nr.	TS5052000
storage temp.	-20°C
Löslichkeit	H₂O: soluble
Aggregatzustand	lyophilized powder
Farbe	white
Wasserlöslichkeit	Soluble in water.

Sicherheit

Kennzeichnung gefährlicher	B,Xn
R-Sätze:	36/37/38-42
S-Sätze:	23-24-26-36/37-22
WGK Germany	3
F	3-10

Gefahreninformationscode (GHS)

Elastase Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
R42:Sensibilisierung durch Einatmen möglich.
S-Sätze Betriebsanweisung:
S23:Gas/Rauch/Dampf/Aerosol nicht einatmen(geeignete Bezeichnung(en) vom Hersteller anzugeben).
S24:Berührung mit der Haut vermeiden.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36/37:Bei der Arbeit geeignete Schutzhandschuhe und Schutzkleidung tragen.
S22:Staub nicht einatmen.
Verwenden
Elastase is used in combination with other proteases for protein analysis by mass spectrometry. Elastase, is used in tissue dissociation because elastin is found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues that contain extensive intercellular fiber networks. It is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin, membrane protein solubilization, protein sequence studies.
Verwenden
Elastase from porcine pancreas has been used in a study to identify a novel bone/calcium metabolism-regulating factor in porcine pancreas. Elastase from porcine pancreas has also been used in a study to investigate the effect of a specific synthetic inhibitor of neutrophil elastase (ONO-5046) on the course of acute hemorrhagic pancreatitis in dogs.
Verwenden
Elastase from porcine pancreas has been used:
- to treat vero cells?to study its effects on syncytium formation
- as a positive control in protease assays
- as a component in RPMI 1640 to isolate human aortic smooth muscle cells (HASMCs) from the aortic tissue
Allgemeine Beschreibung
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.
Biochem/physiol Actions
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.

Elastase (Schweine Pankreas) Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

39445-21-1, Elastase Verwandte Suche:

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