Sulfo-NHS-Biotin Struktur

Sulfo-NHS-Biotin

Bezeichnung:Sulfo-NHS-Biotin

CAS-Nr119616-38-5

Englisch Name:Sulfo-NHS-Biotin

CBNumberCB8277468

SummenformelC14H18N3NaO8S2

Molgewicht443.43

MOL-Datei119616-38-5.mol

Sulfo-NHS-Biotin physikalisch-chemischer Eigenschaften

storage temp.	-20°C
Löslichkeit	H₂O: 10 mg/mL
Aggregatzustand	powder
CAS Datenbank	119616-38-5(CAS DataBase Reference)

Sicherheit

Kennzeichnung gefährlicher	Xn
R-Sätze:	20/21/22-36/37/38-63
S-Sätze:	22-26-36
WGK Germany	3
HS Code	29349990

Gefahreninformationscode (GHS)

Sulfo-NHS-Biotin Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R20/21/22:Gesundheitsschädlich beim Einatmen,Verschlucken und Berührung mit der Haut.
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
R63:Kann das Kind im Mutterleib möglicherweise schädigen.
S-Sätze Betriebsanweisung:
S22:Staub nicht einatmen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
Beschreibung
Sulfo-NHS-Biotin is a linker containing a sulfo-NHS moiety and a biotin group. Sulfo-NHS enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates for conjugation with primary amines. The terminal biotinylation can react with amine molecules in the presence of activator EDC or HATU.
Chemische Eigenschaften
1.3
Verwenden
apoptosis probe, cell permeable fluorescent Ca2+-sensitive dye for cystolic Ca2+ assays
Verwenden
Sulfo-NHS-Biotin is a biotinylation reagent used for biotinylating antibody or protein that can then be used for protein detection or immobilization studies when used in conjunction with streptavidin and immobilized streptavidin. Sulfo-NHS-Biotin was shown to work most effectively when targeting cell surface proteins.
Allgemeine Beschreibung
Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.
Biologische Aktivität
sulfo-nhs-biotin (n-hydroxysulfosuccinimidobiotin) is an amine-reactive biotinylation reagent. the most common amine-reactive biotinylation reagents can be divied into two basic types, n-hydroxysuccinimide (nhs) esters and carboxylates. generally, nhs esters biotinylation reagent contains a reactive nhs ring structure that reacts with an amine on the carbonyl group of a protein or other molecules through nucleophilic attack subsequently forming a stable amide linkage and releasing the nhs group. due to the insolubility of nhs-biotin in aqueous environments, sulfo-nhs-biotin, a water-soluble analog of nhs-biotin, has been developed by attaching a negatively charged sulfonate group on the nhs ring structure, which can be added directly to aqueous reactions without the need for organic solvent dissolution.bioconjugate techniques , 2nd ed. by greg t.hermanson (pierce biotechnology, thermo fisher scientific, rockford, il). academic press (an imprint of elsevier): london, amsterdam, burlington, san diego . 2008. isbn 978-0-12-370501-3.

Sulfo-NHS-Biotin Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

119616-38-5, Sulfo-NHS-Biotin Verwandte Suche:

Send Inquriy