Collagenase + protease inhibitor Struktur

Collagenase + protease inhibitor

Bezeichnung:Collagenase + protease inhibitor

CAS-Nr

Englisch Name:Collagenase + protease inhibitor

CBNumberCB82090407

Molgewicht0

MOL-DateiMol file

Collagenase + protease inhibitor physikalisch-chemischer Eigenschaften

storage temp.

−20°C

Sicherheit

Kennzeichnung gefährlicher	Xn
R-Sätze:	20/21/22-42/43
S-Sätze:	26-36

Gefahreninformationscode (GHS)

Collagenase + protease inhibitor Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R20/21/22:Gesundheitsschädlich beim Einatmen,Verschlucken und Berührung mit der Haut.
R42/43:Sensibilisierung durch Einatmen und Hautkontakt möglich.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
Verwenden
Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets. It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.
Biochem/physiol Actions
Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca2+) per mole of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn2+ is required for activity. This product is used if the collagenase does not require a significant protease activity.

Collagenase + protease inhibitor Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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