MES monohydrate

Bezeichnung:MES monohydrate

CAS-Nr145224-94-8

Englisch Name:MES monohydrate

CBNumberCB7485620

SummenformelC6H15NO5S

Molgewicht213.25

MOL-Datei145224-94-8.mol

MES monohydrate physikalisch-chemischer Eigenschaften

Schmelzpunkt	>300 °C(lit.)
Dichte	10.66g/mL
chüttdichte	570kg/m3
storage temp.	Sealed in dry,Room Temperature
Löslichkeit	H₂O: 0.5 M at 20 °C, clear
Aggregatzustand	Powder, Crystals or Flakes
Farbe	White to pale yellow and/or orange
PH	2.5-4.0 (25℃, 0.5M in H2O)
pka	6.1(at 25℃)
Säure-Base-Indikators(pH-Indikatoren)	5.5 - 6.7
Geruch (Odor)	Odorless
Biologische Quelle	synthetic
Wasserlöslichkeit	Soluble in water.
maximale Wellenlänge (λmax)	λ: 260 nm Amax: 0.025 λ: 280 nm Amax: 0.020
Merck	14,5902
BRN	141319
InChI	InChI=1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
InChIKey	MIIIXQJBDGSIKL-UHFFFAOYSA-N
SMILES	S(=O)(=O)(O)CCN1CCOCC1.O
CAS Datenbank	145224-94-8(CAS DataBase Reference)

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38
S-Sätze:	26-36-37/39
WGK Germany	3
RTECS-Nr.	QE3479500
TSCA	Yes
HS Code	29349990

Gefahreninformationscode (GHS)

MES monohydrate Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S37/39:Bei der Arbeit geeignete Schutzhandschuhe und Schutzbrille/Gesichtsschutz tragen.
Beschreibung
MES Monohydrate is a zwitterionic buffer that is not absorbed through cell membranes and is virtually transparent in UV light. MES is a buffering agent used in many biological and biochemical applications. It is also used as a running buffer for denaturing gel electrophoresis. The characteristics of low UV absorptivity, minimal reactivity, stable pH and solubility in water allow MES Monohydrate to be used as a Good's buffer.
Chemische Eigenschaften
white crystalline powder
Verwenden
MES Monohydrate has been used:
- To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay
- As a buffering agent to stabilize enzymatic solutions
- As a component of PAGE running buffer
Verwenden
MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.
MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH. Alternatively, MES free acid or MES free acid monohydrate can be titrated with sodium hydroxide to attain desired pH.
Verwenden
MES monohydrate is a biological buffer often referred to as Good?s buffer. It is useful for cell culture media and protein based buffer formulations to maintain a stable environment in solution. It is also used in the purification bioprocesses of antibodies, peptides, proteins, blood components and growth factors. It is a useful resolving agent for very small proteins on bis-tris gels. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical applications.
Application
MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.
MES Monohydrate has been used:
To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay.
As a buffering agent to stabilize enzymatic solutions.
As a component of PAGE running buffer.
Allgemeine Beschreibung
buffer substance MES
Preparation and handling
A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
Vorsichtsmaßnahmen
Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.

MES monohydrate Upstream-Materialien And Downstream Produkte

MES monohydrate Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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