LUMI-LIGHTPLUS WESTERN-BLOTTING SUBSTRATE

LUMI-LIGHTPLUS WESTERN-BLOTTING SUBSTRATE Chemische Eigenschaften,Einsatz,Produktion Methoden

• Verwenden

  Lumi-Light^PLUS Western Blotting Substrate has been used in western blotting.

• Allgemeine Beschreibung

  As the technology for quantifying chemiluminescence advances, the substrates for chemiluminescence must also advance. For instance, the light emission of conventional enhanced luminol substrates substantially decreases within 30 minutes. In contrast, the chemiluminescence signal of the Lumi-LightPLUS substrate persists for more than nine hours, and since exposures of only a few minutes are necessary, multiple exposures can be taken. Additionally, the ability to take extended exposures allows the detection of rare proteins, or higher dilutions of antigen or antibody to be used. The emission wavelength of the Lumi-LightPLUS substrate is 425 nm.
  
  Comparison of Lumi-Light Substrate and Lumi-Light PLUS Substrate
  
  Lumi-Light Substrate Lumi-Light^PLUS Substrate
  Sensitivity 10-50 pg antigen 1-5 pg antigen
  Pimary Antibody Dilution Dilute up to 10-fold more compared to colorimetric detection Dilute up to 100-fold more compared to colorimetric detection
  Signal Stability >3 hours (enhance signal with a 30-minute incubation) > 9 hours (enhance signal with a 30-minute incubation)
  
  Number of tests: The reagent is sufficient for 10 blots, each with a size of 10 cm x 10 cm.
  Sensitivity: Depending on the affinity of the primary antibody, 1 - 5 pg of antigen can be detected in research studies.
  Storage/stability: The Lumi-LightPLUS substrate components are stable at +2 to +8°C until the expiration date printed on the label. Components are shipped at +15 to +25°C.

LUMI-LIGHTPLUS WESTERN-BLOTTING SUBSTRATE Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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