2-(4-Iodophenyl)-3-(4-nitrophe-nyl)-5-phenyl-tetrazoliumchlorid

Bezeichnung:2-(4-Iodophenyl)-3-(4-nitrophe-nyl)-5-phenyl-tetrazoliumchlorid

CAS-Nr146-68-9

Englisch Name:Iodonitrotetrazolium chloride

CBNumberCB7232772

SummenformelC19H13ClIN5O2

Molgewicht505.7

MOL-Datei146-68-9.mol

Synonyma

2-(4-Iodphenyl)-3-(4-nitrophenyl)-5-phenyltetrazoliumchlorid

2-(4-Iodophenyl)-3-(4-nitrophe-nyl)-5-phenyl-tetrazoliumchlorid

2-(4-Iodophenyl)-3-(4-nitrophe-nyl)-5-phenyl-tetrazoliumchlorid physikalisch-chemischer Eigenschaften

Schmelzpunkt	240 °C (dec.)(lit.)
storage temp.	2-8°C
Löslichkeit	methanol: water (1:1): 50 mg/mL hot, very faintly turbid, very deep yellow
Aggregatzustand	Powder, Crystals and/or Chunks
Farbe	White to off-white
Wasserlöslichkeit	soluble
Sensitive	Light Sensitive
maximale Wellenlänge (λmax)	248nm
BRN	4093224
Stabilität	Stable. Incompatible with strong oxidizing agents.
Biologische Anwendungen	Bacterial vaginosis diagnosis assay; dehydrogenase enzyme assay; diagnostic assay; food and beverage analytes assays; microbial growth assay; detecting bacteria,yeast,fungi,gamma-hydroxybutyric acid (GHB),microbial growth; measuring niacin,bacterial respiratory activity,superoxide dismutase;treating cancer
InChI	InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
InChIKey	JORABGDXCIBAFL-UHFFFAOYSA-M
SMILES	[N+]1(=NC(C2C=CC=CC=2)=NN1C1C=CC(I)=CC=1)C1=CC=C([N+]([O-])=O)C=C1.[Cl-]
CAS Datenbank	146-68-9(CAS DataBase Reference)
EPA chemische Informationen	2H-Tetrazolium, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-, chloride (146-68-9)

Sicherheit

Kennzeichnung gefährlicher	Xn,F,Xi
R-Sätze:	20/21/22-68/20/21/22-36/37/38-11
S-Sätze:	36/37-36/37/39-26-22-16
RIDADR	2811
WGK Germany	3
F	3-8-10
TSCA	Yes
HazardClass	6.1
PackingGroup	III
HS Code	29339900

Gefahreninformationscode (GHS)

Iodonitrotetrazolium chloride Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R20/21/22:Gesundheitsschädlich beim Einatmen,Verschlucken und Berührung mit der Haut.
R68/20/21/22:Gesundheitsschädlich: Möglichkeit irreversiblen Schadens durch Einatmen,Berührung mit der Haut und durch Verschlucken.
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
R11:Leichtentzündlich.
S-Sätze Betriebsanweisung:
S36/37:Bei der Arbeit geeignete Schutzhandschuhe und Schutzkleidung tragen.
S36/37/39:Bei der Arbeit geeignete Schutzkleidung,Schutzhandschuhe und Schutzbrille/Gesichtsschutz tragen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S22:Staub nicht einatmen.
S16:Von Zündquellen fernhalten - Nicht rauchen.
Beschreibung
Iodonitrotetrazolium (INT) (chloride) is a monotetrazolium salt used as an indicator dye. It is reduced to an insoluble formazan that is used as a vital dye or indicator of cellular redox activity. Reduction commonly results from the activity of dehydrogenases, although non-enzymatic electron transfer reactions can occur in the presence of an intermediate electron acceptor. INT is commonly used to measure the respiratory activity of microorganisms in a variety of contexts.
Chemische Eigenschaften
slightly yellow powder
Verwenden
Iodonitrotetrazolium chloride (INT) on reduction gets converted to iodonitrotetrazolium formazan, a water insoluble violet colored dye or indicator. Hence INT can be used for the colorimetric assay of various dehydrogenases.
Verwenden
Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.
Verwenden
Electron acceptor for the colorimetric assay of various dehydrogenases.
Definition
ChEBI: An organic chloride salt having iodonitrotetrazolium as the counterion.
Sicherheit(Safety)
Iodonitrotetrazolium chloride could cause skin irritation, serious eye irritation, and may cause respiratory irritation.
Properties and Applications
INT (Iodonitrotetrazolium chloride), similar to tetrazolium chloride on reduction, produces a red formazan dye that can be used for quantitative redox assays. It is also toxic to prokaryotes.INT is an artificial electron acceptor that can be utilized in a colorimetric assay to determine protein concentration in a solution. It can be reduced by succinate dehydrogenase to furazan, the formation of which can be measured by absorbance at 490 nm. The activity of succinate dehydrogenase is readily observed by the naked eye as the solution turns from colorless to rusty red.
läuterung methode
Recrystallise the chloride from H2O, aqueous EtOH or EtOH/Et2O. Alternatively, dissolve it in the minimum volume of EtOH and add Et2O; or dissolve it in hot H2O (charcoal), filter and precipitate it by adding conc HCl. Filter the solid off and dry it at 100o. Its solubility in H2O at 25o is 0.5%, and in hot MeOH/H2O (1:1) it is 5%. [Fox & Atkinson J Am Chem Soc 72 3629 1950, Beilstein 26 III/IV 1776.]

2-(4-Iodophenyl)-3-(4-nitrophe-nyl)-5-phenyl-tetrazoliumchlorid Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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