3,3'-Diaminobenzidine tetrahydrochloride hydrate

Bezeichnung:3,3'-Diaminobenzidine tetrahydrochloride hydrate

CAS-Nr868272-85-9

Englisch Name:3,3'-Diaminobenzidine tetrahydrochloride hydrate

CBNumberCB6874674

SummenformelC12H17ClN4O

Molgewicht268.75

MOL-Datei868272-85-9.mol

3,3'-Diaminobenzidine tetrahydrochloride hydrate physikalisch-chemischer Eigenschaften

Schmelzpunkt	300°C
storage temp.	room temp
Löslichkeit	H2O: soluble50mg/mL
Aggregatzustand	Powder
Farbe	White to Amber to Dark purple
Wasserlöslichkeit	Soluble at 5%(w/v) in water

Sicherheit

Kennzeichnung gefährlicher	Xn,T
R-Sätze:	45-68-36-22
S-Sätze:	22-24/25-45-53-26
WGK Germany	3
RTECS-Nr.	DV8753000
TSCA	Yes
HS Code	29215900

Gefahreninformationscode (GHS)

3,3'-Diaminobenzidine tetrahydrochloride hydrate Chemische Eigenschaften,Einsatz,Produktion Methoden

S-Sätze Betriebsanweisung:
S22:Staub nicht einatmen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
Chemische Eigenschaften
Grey to brownish-purple crystalline powder
Verwenden
3,3'-diaminobenzidine-tetrahydrochloride-dihydrate(DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.
Verwenden
3,3′-Diaminobenzidine tetrahydrochloride hydrate can be used in HRP-based assays suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).
Allgemeine Beschreibung
3,3′-Diaminobenzidine tetrahydrochloride hydrate, also, known as DAB HCl is designed as an immunohistology stain to visualize peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.
Biochem/physiol Actions
In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.

3,3'-Diaminobenzidine tetrahydrochloride hydrate Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

868272-85-9, 3,3'-Diaminobenzidine tetrahydrochloride hydrate Verwandte Suche:

Send Inquriy