Triton X-100

Bezeichnung:Triton X-100

CAS-Nr9002-93-1

Englisch Name:Triton X-100

CBNumberCB6682291

SummenformelC18H28O5

Molgewicht324.41192

MOL-Datei9002-93-1.mol

Triton X-100 physikalisch-chemischer Eigenschaften

Schmelzpunkt	44-46 °C
Siedepunkt	250 °C(lit.)
Dichte	1.06 g/mL at 20 °C
Dampfdichte	>1 (vs air)
Dampfdruck	< 1.33 hPa at 20 °C
Brechungsindex	n20/D 1.491
Flammpunkt	535 °F
storage temp.	protect from light
Löslichkeit	H₂O: 0.005 M at 20 °C, clear, colorless
Aggregatzustand	Viscous Liquid
Farbe	≤100(APHA)
PH	6.5-8.5 (25℃)
Geruch (Odor)	Odorless
Säure-Base-Indikators(pH-Indikatoren)	9.7
Wasserlöslichkeit	Miscible with water.
Merck	14,6761
BRN	2315025
LogP	4.610 (est)
EPA chemische Informationen	Octoxynol 9 (9002-93-1)

Sicherheit

Kennzeichnung gefährlicher	Xi,Xn,N
R-Sätze:	41-36/38-22-51/53-36-36/37/38-37/38-52/53
S-Sätze:	26-39-36-61-36/39-36/37/39
RIDADR	UN 3082 9/PG 3
WGK Germany	3
RTECS-Nr.	WZ4750000
F	3
TSCA	Yes
HazardClass	9
PackingGroup	III
HS Code	34021300
Toxizität	LD50 oral in rat: 1800mg/kg

Gefahreninformationscode (GHS)

Triton X-100 Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R41:Gefahr ernster Augenschäden.
R36/38:Reizt die Augen und die Haut.
R22:Gesundheitsschädlich beim Verschlucken.
R51/53:Giftig für Wasserorganismen, kann in Gewässern längerfristig schädliche Wirkungen haben.
R36:Reizt die Augen.
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
R37/38:Reizt die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S39:Schutzbrille/Gesichtsschutz tragen.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S61:Freisetzung in die Umwelt vermeiden. Besondere Anweisungen einholen/Sicherheitsdatenblatt zu Rate ziehen.
S36/39:Bei der Arbeit geeignete Schutzkleidung und Schutzbrille/Gesichtsschutz tragen.
S36/37/39:Bei der Arbeit geeignete Schutzkleidung,Schutzhandschuhe und Schutzbrille/Gesichtsschutz tragen.
Beschreibung
Triton X-100 is a nonionic surfactant that has a hydrophilic polyethylene oxide chain (on average it has 9.5 ethylene oxide units) and an aromatic hydrocarbon lipophilic or hydrophobic group. The hydrocarbon group is a 4-(1,1,3,3-tetramethylbutyl)-phenyl group. Triton X-100 is closely related to IGEPAL CA-630 or former Nonidet P-40, which might differ from it mainly in having slightly shorter ethylene oxide chains. Thus Triton X-100 is slightly more hydrophilic than Igepal CA-630; these two detergents may not be considered to be functionally interchangeable for most applications.
Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Chemische Eigenschaften
Triton X-100 is a very stable material, assumed to be stable for years if stored sealed. It is a clear to slightly hazy, colorless to light yellow liquid (color by APHA = 100).
1. Specific gravity: 1.065 at 25°C (Approx. 1.07 g/mL).
2. Approximate molecular weight = 625, giving effective molarity = 1.7 M for the neat liquid.
3. UV absorption: lambda max = 275 nm and 283 nm in methanol.
4. Viscosity (Brookfield): 240 cps at 25°C.
5. pH (5% aqueous solution): 6.0 to 8.0.
6. Critical micelle concentration (CMC): 0.22 to 0.24 mM.
Verwenden
Nonionic detergent, emulsifier, dispersing agent. Octoxynol-9 as spermaticide.
Verwenden
Detergent, equivalent to Nonidet P-40.Triton X-100 is used as a non-ionic detergent, emulsifier and dispersing agent. It is useful for isolation of membrane proteins. It is also used in electrophoresis. It is an active ingredient in influenza vaccine (Fluzone). It finds applications in the dispersion of carbon materials for soft composite materials and restricting colony expansion in Aspergillus nidulans in microbiology. Further, it is an active ingredient in homemade vinyl record cleaning fluids together with distilled water and isopropyl alcohol.
Verwenden
Triton-X 100 is a non-ionic surfactant. Used in the enhancement of film porosity in conducting polymers.
Definition
ChEBI: A poly(ethylene glycol) terminated with a 4-(2,4,4-trimethylpentan-3-yl) phenyl group at one end.
Trademarks
Triton X-100 (Union Carbide).
Allgemeine Beschreibung
Triton X-305 is a non-ionic surfactant. This octylphenol ethoxylate has higher number of ethylene oxide units, increased cmc (Critical micelle concentration) and hydrophile/lipophile balance when compared to Triton X-100. Triton X-305 is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic and alkaline solutions.
Triton X-100 is a nonionic detergent, 100% active ingredient, which is often used in biochemical applications to solubilize proteins. Triton X-100 has no antimicrobial properties. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It does absorb in the ultraviolet region of the spectrum, however, so can interfere with protein quantitation. A number of polymeric resins have been used to remove X-100 from solution, including Amberlite hydrophobic XAD resins and Rezorian A161 cartridges. The "X" series of Triton detergents are produced from octylphenol polymerized with ethylene oxide. The number ("-100") relates only indirectly to the number of ethylene oxide units in the structure. X100 has an "average of 9.5" ethylene oxide units per molecule, with an average molecular weight of 625. In addition, lower and higher mole adducts will be present in lesser amounts, varying slightly within supplier's standard manufacturing conditions. No antioxidants are added by Sigma or the manufacturer, but commercial preparations of Triton X-100 have been found to contain peroxides up to 0.22% hydrogen peroxide (H2O2) equivalents. These impurities may interfere with biological reactions. Sigma offers X-100-PC and X-100R-PC as biological grade alternatives.
Hazard
Triton X-100 is irritating to human skin and eyes, harmful if ingested and acutely toxic. It caused an increase in cellular and humoral immune responses in a 3-day intermittent skin study in mice, but there was no evidence of teratogenicity in rats.
Biologische Aktivität
Triton X-100 causes a change in the fluorescence characteristics of the Tritoln X-100 effectively ruptures chloroplasts and reduces the electron flow required for the Hill reaction at low concentrations. Treatment of pea root cell preparations with Tritoln X-100 and low-speed centrifugation resulted in a pellet containing a large amount of stromal material (including RNA polymerase and DNA polymerase activity, most of the DNA, about 30% of the RNA, and 50% of the protein)^[1-2].
Sicherheitsprofil
Moderately toxic by ingestion and intravenous routes. Experimental reproductive effects. Human mutation data reported. An eye and human skin irritant. Many glycol ethers cause dangerous human reproductive effects. When heated to decomposition it emits toxic fumes of NOx. A surfactant. See also GLYCOL ETHERS.
Einzelnachweise
[1] L P VERNON; E S. Photochemical Activities of Spinach Chloroplasts Following Treatment with the Detergent Triton X-100.[J]. Plant Physiology, 1965. DOI:10.1104/pp.40.6.1269.
[2] BOTTOMLEY W. Some effects of triton x-100 on pea etioplasts.[J]. Plant Physiology, 1970. DOI:10.1104/pp.46.3.437.

Triton X-100 Upstream-Materialien And Downstream Produkte

Triton X-100 Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

9002-93-1, Triton X-100 Verwandte Suche:

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