3,3',5,5'-Tetramethylbenzidin

Bezeichnung:3,3',5,5'-Tetramethylbenzidin

CAS-Nr54827-17-7

Englisch Name:Tetramethylbenzidine

CBNumberCB6682185

SummenformelC16H20N2

Molgewicht240.34

MOL-Datei54827-17-7.mol

Synonyma

3,3',5,5'-Tetramethylbenzidin

3,3',5,5'-Tetramethylbenzidin physikalisch-chemischer Eigenschaften

Schmelzpunkt	168-171 °C(lit.)
Siedepunkt	100 °C
Dichte	1
Brechungsindex	1.5519 (estimate)
storage temp.	2-8°C
Löslichkeit	Slightly soluble. <0.1 g/100 mL at 20°C.
pka	4.49±0.10(Predicted)
Aggregatzustand	tablet
Farbe	Cream
Wasserlöslichkeit	Slightly soluble. <0.1 g/100 mL at 20 ºC
Sensitive	Light Sensitive
BRN	2808541
Stabilität	Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents.
CAS Datenbank	54827-17-7(CAS DataBase Reference)
EPA chemische Informationen	3,3',5,5'-Tetramethylbenzidine (54827-17-7)

Sicherheit

Kennzeichnung gefährlicher	Xi,Xn
R-Sätze:	36/38-36/37/38-22-41
S-Sätze:	26-36-36/37-39
RIDADR	UN 2796 8/PG 2
WGK Germany	3
RTECS-Nr.	DV2300000
F	8-10
TSCA	Yes
HazardClass	6.1
HS Code	29215990
Giftige Stoffe Daten	54827-17-7(Hazardous Substances Data)

Gefahreninformationscode (GHS)

Tetramethylbenzidine Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/38:Reizt die Augen und die Haut.
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
R22:Gesundheitsschädlich beim Verschlucken.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S36/37:Bei der Arbeit geeignete Schutzhandschuhe und Schutzkleidung tragen.
Beschreibung
Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). It is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights. It is a soluble chromogen substrate for horseradish peroxidase detection systems. Tetramethylbenzidine is recommended for ELISA procedures.
Chemische Eigenschaften
Tetramethylbenzidine is a white or light yellow solid, odorless, tasteless, insoluble in water, easily soluble in acetone, ether, dimethyl sulfoxide, dimethylformamide and other organic solvents. It forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights.
Verwenden
3,3,5,5-Tetramethyl benzidine is used as a reagent in a sensitive staining procedure for the detection of low levels of heme-associated peroxidase activity of cytochrome P-450 on SDS-polyacrylamide or agarose gel; non-carcinogenic substitute for benzidine as reagent for the detection of blood and determination of hemoglobin content.
synthetische
The synthesis of 3,3',5,5'-Tetramethylbenzidine involved using 2,6-dimethylaniline as the raw material. The process included activation, oxidative coupling, and purification, resulting in the production of pure material. The yield of 3,3,5,5-tetramethylbenzidine was 65%.
Reaktionen
TMB can act as a hydrogen donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as horseradish peroxidase.

Shows the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to 3,3',5,5'-tetramethylbenzidine diimine
The resulting diimine causes the solution to take on a blue colour, and this colour change can be read on a spectrophotometer at the wavelengths of 370 and 650 nm.
Allgemeine Beschreibung
3,3',5,5'-tetramethylbenzidine appears as pale yellow crystals or off-white powder. (NTP, 1992)
Air & Water Reaktionen
Insoluble in water.
Reaktivität anzeigen
Tetramethylbenzidine is sensitive to prolonged exposure to light . Neutralizes acids in exothermic reactions to form salts plus water. May be incompatible with isocyanates, halogenated organics, peroxides, phenols (acidic), epoxides, anhydrides, and acid halides. Flammable gaseous hydrogen may be generated in combination with strong reducing agents, such as hydrides.
Brandgefahr
Flash point data for Tetramethylbenzidine are not available, however, Tetramethylbenzidine is probably combustible.
Biochem/physiol Actions
3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in?situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.
Carcinogenicity
The carcinogenic potential of Tetramethylbenzidine is uncertain due to conflicting evidence. It is not mutagenic by the Ames test, and did not induce formation of tumors in a single-arm study of 24 rats. On that evidence, it has been used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.
DOI: 10.1177/26.2.24068

Tetramethylbenzidine Upstream-Materialien And Downstream Produkte

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