Dehydrogenase, Glycerinaldehydphosphat

Bezeichnung:Dehydrogenase, Glycerinaldehydphosphat

CAS-Nr9001-50-7

Englisch Name:GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE

CBNumberCB6673129

SummenformelC3H7O6P

Molgewicht170.057841

MOL-Datei9001-50-7.mol

Synonyma

Dehydrogenase, Glycerinaldehydphosphat

Dehydrogenase, Glycerinaldehydphosphat physikalisch-chemischer Eigenschaften

storage temp.	-20°C
Aggregatzustand	suspension
Farbe	white
EPA chemische Informationen	Dehydrogenase, glyceraldehyde phosphate (9001-50-7)

Sicherheit

Kennzeichnung gefährlicher	B,Xi
R-Sätze:	36/37/38
S-Sätze:	26-36
WGK Germany	3

Gefahreninformationscode (GHS)

GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden
Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:
- for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
- to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
- in anti-aggregation assays
- in purified GAPDH studies
Allgemeine Beschreibung
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway. GAPDH has also been found to function in additional cellular process, such as transcription, apoptosis, oxidative stress and ER to Golgi transport.
Biochem/physiol Actions
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<,New>>>.
läuterung methode
Purify the dehydrogenase from rabbit muscle by extraction with 0.03N KOH and precipitate it with (NH4)2SO4 (0.52 of saturation). The clear supernatant is adjusted to pH 7.5, and NH3 is added dropwise to pH 8.2-8.4. Crystals appear sometimes even without seeding. The crystals are dissolved in H2O, filtered to remove suspended material and 2 volumes of saturated (NH4)2SO4 at pH 8.2-8.4 is added. After 1hour the crystals appear. Recrystallise it in the same way. [Cori et al. J Biol Chem 173 605 1948, Furfine & Velick J Biol Chem 240 844 1965, The Enzymes 7 243 1963, Lui & Huskey Biochemistry 31 6998 1992.] The Km values are: NADH (3.3WM) and 1,3-diphosphoglycerate (8x10-7M) in pH 7.4 imidazole buffer at 26o, NAD (13WM), glyceraldehyde-3-P (90WM), Pi (2.9x10-4M), and arsenate (69WM) in 8.6 M NaHCO3 buffer at 26oC. [Orsi & Cleland Biochemistry 11 102 1972.]

Dehydrogenase, Glycerinaldehydphosphat Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

9001-50-7, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE Verwandte Suche:

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