Neticonazole (10 μM; 48 hours; C4-2B cells) treatment decreases the levels of both Alix and Rab27a, and significantly decreases nSMase2 levels. Neticonazole causes a significant inhibition in p-ERK levels.
Neticonazole (0-10 μM) exhibits a potent and dose-dependent inhibition of exosome release from C4-2B cells.
It is also an orally active exosome biogenesis and secretion inhibitor.
Western Blot Analysis
| Cell Line: | C4-2B cells | td>
| Concentration: | 10 μM |
| Incubation Time: | 48 hours |
| Result: | Deccreased the levels of both Alix and Rab27a, and significantly decreased nSMase2 levels. |
in vivo
Neticonazole (1-100 ng/kg; oral gavage; daily; for 15 days; male C57BL/6 mice) treatment significantly improves the survival of intestinal dysbacteriosis (IDB) mice with colorectal cancer (CRC) xenograft tumors , likely through increasing apoptosis of CRC xenograft tumor cells.
| Animal Model: | Male C57BL/6 mice (8 weeks old) given ampicillin , neomycin, metronidazole and vancomycin, and injected with SW480 cells |
| Dosage: | 1 ng/kg , 10 ng/kg and 100 ng/kg |
| Administration: | Oral gavage; daily; for 15 days |
| Result: | Significantly improved the survival of IDB mice with CRC xenograft tumors. | td>
target
