Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*

Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* Chemische Eigenschaften,Einsatz,Produktion Methoden

• Verwenden

  For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation.
  
  • Biotin-16-dUTP is used as asubstrate for: Terminal Transferase
  • DNA polymerase I (holoenzyme and Klenow fragment)
  • Taq DNA polymerase
  • Reverse transcriptase (e.g., Transcriptor)
  Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.
  The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling.
  
  • Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate
  • Biotin Luminescence Detection Kit

• Allgemeine Beschreibung

  Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases.

• Biochem/physiol Actions

  Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency.

Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global(9)Suppliers