3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-ar-carbonsure

Bezeichnung:3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-ar-carbonsure

CAS-Nr72088-94-9

Englisch Name:5(6)-Carboxyfluorescein

CBNumberCB5257173

SummenformelC42H24O14

Molgewicht752.63

MOL-Datei72088-94-9.mol

Synonyma

3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-ar-carbonsure

3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-ar-carbonsure physikalisch-chemischer Eigenschaften

Schmelzpunkt	275 °C
Siedepunkt	464.9°C (rough estimate)
Dichte	1.3418 (rough estimate)
Brechungsindex	1.6380 (estimate)
storage temp.	Keep in dark place,Inert atmosphere,Room temperature
Löslichkeit	DMSO (Sparingly), Methanol (Slightly, Heated)
Aggregatzustand	Solid
Farbe	Light Yellow to Dark Brown
Wasserlöslichkeit	slightly soluble
maximale Wellenlänge (λmax)	492 nm
ε(Extinktionskoeffizient)	12134 at 284nm in 0.1 M NaOH at 0.005g/L 43060 at 239nm in 0.1 M NaOH at 0.005g/L
InChIKey	CPRVUBZYHZGASQ-UHFFFAOYSA-N
SMILES	C12(OC(=O)C3C=CC(C(=O)O)=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1.C12(OC(=O)C3C=C(C(=O)O)C=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1
EPA chemische Informationen	Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo- (72088-94-9)

Sicherheit

Kennzeichnung gefährlicher	Xi,Xn
R-Sätze:	36-36/37/38-20/21/22
S-Sätze:	37/39-26-36-22
WGK Germany	3
TSCA	Yes
HS Code	29322090

Gefahreninformationscode (GHS)

5(6)-Carboxyfluorescein Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36:Reizt die Augen.
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
R20/21/22:Gesundheitsschädlich beim Einatmen,Verschlucken und Berührung mit der Haut.
S-Sätze Betriebsanweisung:
S37/39:Bei der Arbeit geeignete Schutzhandschuhe und Schutzbrille/Gesichtsschutz tragen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
Beschreibung
5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers.
Chemische Eigenschaften
Dark-Orange to Brown Solid
Verwenden
5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent.
Verwenden
A popular probe of determination of intracellular pH.
Verwenden
As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent
Definition
ChEBI: 5(6)-carboxyfluorescein is a commercially available mixture of 5-carboxyfluorescein and 6-carboxyfluorescein isomers. A fluorescent dye, it is commonly used as a tracer agent. It has a role as a tracer and a fluorescent dye. It contains a 5-carboxyfluorescein and a 6-carboxyfluorescein.
reaction suitability
reagent type: fluorescent-labelling reagent
Solubility in organics
5(6)-Carboxyfluorescein is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of 5(6)-carboxyfluorescein in these solvents is approximately 5, 0.5, and 1 mg/ml, respectively.

3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-ar-carbonsure Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

72088-94-9, 5(6)-Carboxyfluorescein Verwandte Suche:

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