NEP

NEP Chemische Eigenschaften,Einsatz,Produktion Methoden

• Biologische Aktivität

  NEP (VDP-green (NEP)) is an intramolecular charge transfer (ICT)-based sensing fluorescent probe for sensing orthodithiol-containing proteins (VDPs). NEP exhibits high selectivity for VDPs in living cells and animals, and displays a strong green fluorescence signal (λex/λem=430/535 nm). NEP has potential for Parkinson's research.

• in vitro

  NEP (1-30 μM; 6 hours) has no or little cytotoxicity in HepG2 and PC12 cells.
  NEP (10 μM; 4 hours) causes the fluorescence intensity to decrease gradually in PC12 cells pretreated with 6-OHDA (0, 50, 100, and 200 μM; for 30 min).
  NEP contains a dithiarsolane moiety (five-membered As-S ring) as the receptor of VDPs. In the presence of VDPs, NEP displays a strong green fluorescence signal produced by the cyclic dithiarsolane cleavage and subsequent intramolecular cyclization to liberate the fluorophore. NEP maintains a reliable fluorescence response within the range of pH 7-10.
  

• in vivo

  NEP (10 μM; for 4 h) causes the obvious green signal in zebrafishes (4 day old).
  

NEP Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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