BOHEMINE Struktur

BOHEMINE

Bezeichnung:BOHEMINE

CAS-Nr189232-42-6

Englisch Name:BOHEMINE

CBNumberCB4503170

SummenformelC18H24N6O

Molgewicht340.42

MOL-Datei189232-42-6.mol

BOHEMINE physikalisch-chemischer Eigenschaften

Schmelzpunkt	101-103 °C
Siedepunkt	589.4±60.0 °C(Predicted)
Dichte	1.27±0.1 g/cm3(Predicted)
storage temp.	Keep in dark place,Sealed in dry,2-8°C
Löslichkeit	chloroform: 50 mg/mL, soluble
Aggregatzustand	powder
pka	14.94±0.10(Predicted)

Sicherheit

WGK Germany

Gefahreninformationscode (GHS)

BOHEMINE Chemische Eigenschaften,Einsatz,Produktion Methoden

Definition
ChEBI: Bohemine is purine substituted on C-2, C-6 and N-9 with (3-hydroxypropyl)amino, benzylamino and isopropyl groups respectively; a synthetic, cell-permeable, cyclin-dependent kinase (CDK) inhibitor that is structurally similar to olomoucine and roscovitine. It has a role as an EC 2.7.11.22 (cyclin-dependent kinase) inhibitor.
Biologische Aktivität
a highly conserved array of mechanisms regulating cyclin-dependent kinases (cdk), which govern the timing of cell cycle progression and division, has developed in eukaryotes. cdk inhibitors have been shown to play an key regulatory role in cell proliferation, differentiation, senescence, as well as programmed death.
in vitro
bohemine was applied to cultures of mouse hybridoma cells in order to evaluate its capacity of cell growth and the production of monoclonal antibody. results showed that addition of bohemine at concentrations in the range of 1-10 μm led to a short-term arrest of growth and monoclonal antibody production. such short-term suppression of cell functions was followed by a significant temporary increase of specific growth rate and specific production rate. in semicontinuous cultures, the steady-state viable cell density values showed a certain stimulation of cell growth in the presence of bohemine at micromolar concentrations, and growth inhibition at 10 and 30 μm [1].
in vivo
in mice, bohemine was found to be rapidly and completely metabolized and disappeared from circulation during the first 60 min following i.v. administration. moreover, the metabolites were eliminated by the hepatobiliary tract and also by renal excretion [2].
target

Target Value
Cdk9/cyclin T1
() 2.7 μM
Cdk2/cyclin E
() 4.6 μM
Cdk2/cyclin A
() 83 μM
IC 50
1 μm for cyclin-dependent kinase
Einzelnachweise
[1] franek f,strnad m,havlícek l,siglerová v,fismolová i,eckschlager t. diverse effects of the cyclin-dependent kinase inhibitor bohemine: concentration- and time-dependent suppression or stimulation of hybridoma culture. cytotechnology.2001 jul;36(1-3):117-23.
[2] chmela z,vesel j,lemr k,rypka m,hanus j,havlícek l,krystof v,michnová l,fuksová k,lukes j. in vivo metabolism of 2,6,9-trisubstituted purine-derived cyclin-dependent kinase inhibitor bohemine in mice: glucosidation as the principal metabolic route. drug metab dispos.2001 mar;29(3):326-34.

BOHEMINE Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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Target	Value
Cdk9/cyclin T1 ()	2.7 μM
Cdk2/cyclin E ()	4.6 μM
Cdk2/cyclin A ()	83 μM