YS-49 (1-100 μM; 18 hours; RAVSMC and RAW 264.7 cells) concentration-dependently inhibits the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to lipopolysaccharide (LPS) plus INF-γ, with IC 50 values of 22 μM and 30 μM, respectively.
YS-49 (10-100 μM; 18 hours; RAVSMC and RAW 264.7 cells) suppresses iNOS gene expression induced by LPS and/or cytokines in RAVSMC and RAW 264.7 cells at the transcriptional level.
Cell Viability Assay
| Cell Line: | RAVSMC and RAW 264.7 cells |
| Concentration: | 10 μM, 30 μM and 100 μM (RAVSMC cells); 1 μM, 10 μM and 100 μM (RAW 264.7 cells) |
| Incubation Time: | 18 hours |
| Result: | Inhibited the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to LPS+INF-γ, with IC 50 values of 22 and 30 μM, respectively . |
Western Blot Analysis
| Cell Line: | RAVSMC and RAW 264.7 cells |
| Concentration: | 10 μΜ, 30 μΜ and 100 μΜ |
| Incubation Time: | 18 hours |
| Result: | Concentration -dependently inhibited the expression of iNOS protein induced by LPS plus IFN-γ. |
