KAPA Mouse Genotyping Kit

Gefahreninformationscode (GHS)

• Bildanzeige (GHS)

  

• Alarmwort

  Warnung

• Gefahrenhinweise

  H371：Kann die Organe schädigen.

• Sicherheit

  P260：Dampf/Aerosol/Nebel nicht einatmen.

  P264：Nach Gebrauch gründlich waschen.

  P264：Nach Gebrauch gründlich waschen.

  P270：Bei Gebrauch nicht essen, trinken oder rauchen.

  P405：Unter Verschluss aufbewahren.

  P501：Inhalt/Behälter ... (Entsorgungsvorschriften vom Hersteller anzugeben) zuführen.

KAPA Mouse Genotyping Kit Chemische Eigenschaften,Einsatz,Produktion Methoden

• Verwenden

  KAPA Mouse Genotyping Kit has been used in the DNA extraction and amplification from mouse tail, ear, and toe tissue. The kit is also suitable for extraction and amplification of DNA from other animal tissue, but protocol optimization may be required.

• Allgemeine Beschreibung

  KAPA Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour, as compared to ≥1 day with conventional protocols.KAPA2G Fast Genotyping Mix (2X) is a ready-to-use master mix containing all components for fast PCR, except primers and templates.

• Biochem/physiol Actions

  DNA fragments generated with KAPA2G Fast Genotyping Mix are 3′-dA-tailed and may be cloned into TA cloning vectors, or used for routine downstream analyses or applications, including restriction enzyme digestion, cloning, and sequencing. Like wild-type Taq, KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated.

KAPA Mouse Genotyping Kit Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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