RESORUFIN ETHYL ETHER Struktur

RESORUFIN ETHYL ETHER

Bezeichnung:RESORUFIN ETHYL ETHER

CAS-Nr5725-91-7

Englisch Name:RESORUFIN ETHYL ETHER

CBNumberCB3203954

SummenformelC14H11NO3

Molgewicht241.24

MOL-Datei5725-91-7.mol

RESORUFIN ETHYL ETHER physikalisch-chemischer Eigenschaften

Schmelzpunkt	223-225 °C (lit.)
Siedepunkt	389.3±42.0 °C(Predicted)
Dichte	1.29±0.1 g/cm3(Predicted)
storage temp.	-20°C
Löslichkeit	DMF: soluble
pka	1.43±0.20(Predicted)
Aggregatzustand	Brown solid.
Farbe	Dark Orange to Red
BRN	225973

Sicherheit

WGK Germany	3
F	8-10

Gefahreninformationscode (GHS)

RESORUFIN ETHYL ETHER Chemische Eigenschaften,Einsatz,Produktion Methoden

Chemische Eigenschaften
Red Solid
Verwenden
Resorufin ethyl ether is suitable for the study of dealkylating activity of cytochrome P450 isozymes 1A1 and 1A2, where arylalkyl and alkyl isothiocyanates, and their glutathione, cysteine, and A′-acetylcysteine conjugates are used as inhibitors. It is suitable to study the ethoxyresorufin-O-deethylase (EROD) activity of cytochrome P450 activity (CYP1A1).
Verwenden
A fluorimetric substrate for cytochrome P450 IAI. Inhibits vasorelaxant responses to acetylcholine
Verwenden
Dealkylase substrate for the microfluorimetric analysis of microsomal cytochrome P-450.
Definition
ChEBI: 7-ethoxyresorufin is a phenoxazine.
Allgemeine Beschreibung
Resorufin ethers are used as markers or substrates for different cytochrome P (CYP) isoforms. Resorufin ethyl ether is the preferred resorufin ether to be metabolized by microsomes of 3-methylcholanthrene treated animals.
Biologische Aktivität
cytochrome p450, family 1, subfamily a, polypeptide 1 is a protein that is encoded by human cyp1a1 gene. the protein is a member of the cytochrome p450 superfamily of enzymes. cyp1a1 is involved in phase i xenobiotic and drug metabolism. cyp1a1 is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. 7-ethoxyresorufin is a specific subtrate of cyp1a.
in vitro
7-ethoxyresorufin (2-10 microm), a substrate for cytochrome p450, which binds to the heme moiety of the enzyme, reduced responses to acetylcholine concentration-dependently but not the other agonists indicating an effect on nitric oxide synthesis although neither affected the conversion of l-arginine to l-citrulline [1].
in vivo
activities of cyp1a (7-ethoxyresorufin) in the liver was determined for comparative purposes. in mice, lps depressed cyp2a5 at low doses but not at doses that increased pro-inflammatory cytokines and no serum levels, and depressed cyp1a activity. blockade of proinflammatory cytokines extended down-regulation of cyp2a5 while not affecting lps-induced depression of cyp1a [2].
Lager
Store at -20°C
Einzelnachweise
[1] oyekan ao, mcgiff jc, rosencrantz-weiss p, quilley j. relaxant responses of rabbit aorta: influence of cytochrome p450 inhibitors. j pharmacol exp ther. 1994 jan;268(1):262-9.
[2] de-oliveira ac, poça ks, totino pr, paumgartten fj. modulation of cytochrome p450 2a5 activity by lipopolysaccharide: low-dose effects and non-monotonic dose-response relationship. plos one. 2015 jan 30;10(1):e0117842.
[3] pelkonen o, pasanen m, kuha h, gachalyi b, kairaluoma m, sotaniemi ea, park ss, friedman fk, gelboin hv. the effect of cigarette smoking on 7-ethoxyresorufin o-deethylase and other monooxygenase activities in human liver: analyses with monoclonal antibodies. br j clin pharmacol. 1986 aug;22(2):125-34.

RESORUFIN ETHYL ETHER Upstream-Materialien And Downstream Produkte

RESORUFIN ETHYL ETHER Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

5725-91-7, RESORUFIN ETHYL ETHER Verwandte Suche:

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