Kanamycinsulfat

Bezeichnung:Kanamycinsulfat

CAS-Nr25389-94-0

Englisch Name:Kanamycin sulfate

CBNumberCB2722315

SummenformelC18H38N4O15S

Molgewicht582.58

MOL-Datei25389-94-0.mol

Synonyma

Kanamycinsulfat

Kanamycinsulfat physikalisch-chemischer Eigenschaften

Schmelzpunkt	250°C
alpha	+112～+123°(D/20℃)(c=1,H2O)(calculated on the dried basis)
Dichte	1.3619 (rough estimate)
Brechungsindex	117.5 ° (C=1, H2O)
storage temp.	2-8°C
Löslichkeit	H₂O: 10-50 mg/mL As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 5 days.
Aggregatzustand	powder
Farbe	white to off-white
Säure-Base-Indikators(pH-Indikatoren)	6.5 - 8.5 (1% solution)
Wasserlöslichkeit	Soluble in water at 10mg/ml
Merck	14,5281
InChIKey	OOYGSFOGFJDDHP-KMCOLRRFSA-N

Sicherheit

Kennzeichnung gefährlicher	T
R-Sätze:	61
S-Sätze:	22-24/25-45-53
WGK Germany	3
RTECS-Nr.	NZ3225030
HS Code	29419020
Toxizität	LD50 in mice: 20.7 g/kg orally; 1450 mg/kg i.p. (Zel'tser)

Gefahreninformationscode (GHS)

Kanamycin sulfate Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R61:Kann das Kind im Mutterleib schädigen.
S-Sätze Betriebsanweisung:
S22:Staub nicht einatmen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
S45:Bei Unfall oder Unwohlsein sofort Arzt zuziehen (wenn möglich, dieses Etikett vorzeigen).
Beschreibung
Kanamycin was found by Umezawa et al. in the culture broth of Streptomyces kanamyceticus in 1957. It is produced with other components, kanamycins B (bekanamycin) and C, which are also separated during the purification. The compound shows much lower toxicity than the earlier aminoglycosides, streptomycin and neomycin, and strong activity against a wide range of gram-positive and gram-negative bacteria, including Mycobacterium. Kanamycin has been used clinically for treatment of such serious infections as dysentery, salmonellosis, and tuberculosis.
Chemische Eigenschaften
Off-white powder
Originator
Kantrex,Bristol,US,1958
Verwenden
Kanamycin sulfate is an antibacterial agent similar to neomycin.
Verwenden
Antibiotic complex produced by Streptomyces kanamyceticus Okami & Umezawa from Japanese soil. Comprised of three components, kanamycin A, the major component, and kanamycins B and C, two minor congeners. Antibacterial.
Verwenden
Kanamycin monosulfate, is used as an aminoglycoside antibiotic. Kanamycin can be used as a selection agent in several types of isolation media.Kanamycin sulfate from Streptomyces kanamyceticus has been used in tissue culture media. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100mg/mL.
Definition
A broadspectrum antibiotic.
Manufacturing Process
As described in US Patent 2,931,798, Streptomyces kanamyceticus (K2-J) was first cultured in shake flasks in the following media: (a) 0.75% meat extract, 0.75% peptone, 0.3% NaCl, with 1.0% of starch, dextrin, maltose, glucose, lactose, sucrose or glycerol; or (b) 2.0% soybean meal, 0.05% KCl, 0.05% MgSO4 · 7H2O, 0.5% NaCl, 0.2% NaNO3, with 1.0% of starch, dextrin, maltose, glucose, lactose, sucrose or glycerol. The initial pH of all media was adjusted to 7.0. After 24 to 48 hours shaking in some cases the pH decreased to about 6.0 to 6.8, but from 72 to 120 hours the pH rose and became 7.5 to 8.6. The production of kanamycin was apparent after 48 hours and, depending on the media; the maximum production was found after 72 to 120 hours.
The yield was highest with starch or dextrin, intermediate and about the same with sucrose, glucose, maltose and lactose and poorest with glycerol. Kanamycin was produced by media containing soybean meal, peanut meal, cottonseed meal, corn steep liquor, peptone, yeast extract or meat extract, with or without sodium nitrate. Commercially available soybean meal was recognized to be one of the best nitrogen sources. The addition of corn steep liquor, peptone, yeast extract or nitrate to the soybean meal promoted the production of kanamycin.
The brownish white kanamycin (5 g) was dissolved in 50 ml of 60% aqueous methanol, insoluble material was removed and to the filtrate 40 ml of 60% aqueous methanol containing 2,000 mg of ammonium sulfate was added, and the precipitated kanamycin sulfate was collected, washed with 50 ml of 80% aqueous methanol, and dried. Thus, 4.5 g of kanamycin sulfate was obtained as a light brownish powder.
Trademarks
Klebcil (King).
Therapeutic Function
Antibacterial
Allgemeine Beschreibung
Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 2 mL/L.
Biologische Aktivität
soluble in water at 50mg/ml recommend our ultra pure, nuclease free water, ab02123 typically used at 100mg/ml for cell culture protect from moisture store: 2-8°c.
Biochem/physiol Actions
Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding. Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.
Lager
Room temperature

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