Ribonuclease H (RNase H)

Ribonuclease H (RNase H) Chemische Eigenschaften,Einsatz,Produktion Methoden

• Verwenden

  Ribonuclease H (RNase H) has been used for:
  
  • In vivo RNA-primed initiation of DNA synthesis
  • Elimination of mRNA during second-strand cDNA synthesis
  • Site-specific cleavage of RNA
  • Detection of RNA:DNA regions in double-stranded DNA of natural origin
  • Removal of poly (A) sequences of mRNA if oligo (dT) is present
  • RNA extraction and quantitative reverse transcriptase polymerase chain reaction (RT-PCR)

• Allgemeine Beschreibung

  Nonspecific endoribonuclease that specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides.
  
  Source: E. coli H560 pol A1
  Storage Buffer: 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C)
  Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer.

• Biochem/physiol Actions

  Ribonuclease H (RNase H) specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides. RNase H is associated with nucleic acid immunity. Using RNase H for degrading mRNA results in 80% depletion of mRNA and protein expression. RNase H recognizes the start codon and the 3′ and 5′ untranslated regions. This enzyme participates in DNA replication.

Ribonuclease H (RNase H) Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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