PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming

PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming Chemische Eigenschaften,Einsatz,Produktion Methoden

• Verwenden

  Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:
  
  • in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)
  • to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membrane
  • to cleave immunolabeled HeLa cells
  
  

• Verwenden

  Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.

• Allgemeine Beschreibung

  Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.

• Biochem/physiol Actions

  Used for the release of GPI anchored proteins from the membrane.

PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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