Subtilisin

Bezeichnung:Subtilisin

CAS-Nr9014-01-1

Englisch Name:EC 3.4.21.14

CBNumberCB1670037

SummenformelUnspecified

Molgewicht0

MOL-DateiMol file

Synonyma

Subtilisin

Subtilisin physikalisch-chemischer Eigenschaften

Dichte	1.3
Dampfdruck	0Pa at 25℃
storage temp.	2-8°C
Aggregatzustand	powder
Farbe	white
Wasserlöslichkeit	100g/L at 25℃
Stabilität	store cold
LogP	-3.1 at 25℃
CAS Datenbank	9014-01-1
EPA chemische Informationen	Subtilisin (9014-01-1)

Sicherheit

Kennzeichnung gefährlicher	Xn,N
R-Sätze:	37/38-41-42-36/37/38-50-22
S-Sätze:	23-24-26-36/37/39-22-36/37-61-45-39
RIDADR	3082
WGK Germany	2
RTECS-Nr.	UK9540000
F	3-10
HS Code	35079090
Giftige Stoffe Daten	9014-01-1(Hazardous Substances Data)
Toxizität	LD50 orl-rat: 3700 mg/kg FCTXAV 7,581,69

Gefahreninformationscode (GHS)

EC 3.4.21.14 Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R37/38:Reizt die Atmungsorgane und die Haut.
R41:Gefahr ernster Augenschäden.
R42:Sensibilisierung durch Einatmen möglich.
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S23:Gas/Rauch/Dampf/Aerosol nicht einatmen(geeignete Bezeichnung(en) vom Hersteller anzugeben).
S24:Berührung mit der Haut vermeiden.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36/37/39:Bei der Arbeit geeignete Schutzkleidung,Schutzhandschuhe und Schutzbrille/Gesichtsschutz tragen.
S22:Staub nicht einatmen.
S36/37:Bei der Arbeit geeignete Schutzhandschuhe und Schutzkleidung tragen.
Chemische Eigenschaften
These are proteolytic enzymes which take the form of light-colored, free-flowing powders. A protein containing numerous amino acids
Verwenden
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).
Verwenden
Protease from Bacillus sp. has been used to determine its proteolytic activity by spectrophotometric method.
Verwenden
Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.
Allgemeine Beschreibung
Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.
Biochem/physiol Actions
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typcially stabilized by Ca2+ and have high isoelectric points.
Sicherheitsprofil
Moderately toxic by ingestion. Aneye irritant. When heated to decomposition it emits toxicfumes of NOx.
mögliche Exposition
These commercial proteolytic enzymes are used in laundry detergent formulations
läuterung methode
This alkaline protease is purified 211-fold by affinity chromatography using 4-(4-aminophenylazo)phenylarsonic acid complex to activated CH-Sepharose 4B. It is inhibited by 2-phenylethane boronic acid, PMSF, 3,4-dichloroisocoumarin, acetone and benzamide. [Chandraskaren & Dhar Anal Biochem 150 141 1985, Schomburg & Schomburg Springer Handbook of Enzymes 2nd Edn vol 7 p 286 2002.] Synexin (from bovine liver) M 47,000 Da. This Ca binding protein is purified by (NH4)2SO4 precipitation, then by a specific pH step elution from a chromatofocusing medium in the absence of ampholytes. The pI is 7.5. [Scott et al. Anal Biochem 149 163 1985.]
Inkompatibilitäten
Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides.

Subtilisin Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

9014-01-1, EC 3.4.21.14 Verwandte Suche:

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