Polymerase Chain Reaction

Polymerase Chain Reaction Chemische Eigenschaften,Einsatz,Produktion Methoden

• Beschreibung

  The polymerase chain reaction (PCR) is a method to amplify DNA exponentially by in vitro DNA synthesis (1,2). Amplification usually consists of three steps that are repeated 10 to 50 times in an automated thermal cycler . The first step is thermal denaturation (strand separation) of target DNA to be amplified. The second step is annealing of two oligonucleotide primers to the separated DNA strands. The final step is DNA synthesis by a thermostable DNA polymerase that extends the primers. These newly synthesized DNA strands act as target DNA for subsequent cycles of synthesis. If primers are specific for the flanking ends of a particular DNA sequence, they anneal or base-pair only with that sequence; hence DNA between the primer sequences is amplified to generate millions of DNA copies. This synthetic DNA is visualized as a single band on an ethidium bromide-stained agarose gel. If primers are less specific, they anneal to multiple target sites, and multiple DNA fragments are amplified.

• Verwenden

  PCR is a powerful diagnostic tool that offers several advantages over traditional methods of plant disease diagnosis (2). Primarily, organisms need not be cultured to confirm their presence or identity in diseased plant tissue, since oligonucleotide primers specific for a particular pathogen can be used to amplify pathogen DNA directly from infected plants.
  PCR techniques may also provide DNA or mRNA profiles, or fingerprints, of phytopathogens (9–13). DNA fingerprints are used to distinguish between species and strains, and fingerprinting techniques may be used to generate simple or complex DNA profiles.

Polymerase Chain Reaction Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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