Rp-8-bromo-Cyclic AMPS (sodium salt) Struktur

Rp-8-bromo-Cyclic AMPS (sodium salt)

Bezeichnung:Rp-8-bromo-Cyclic AMPS (sodium salt)

Englisch Name:Rp-8-bromo-Cyclic AMPS (sodium salt)

CBNumberCB04666286

SummenformelC10H12BrN5NaO5PS

Molgewicht448.16

Rp-8-bromo-Cyclic AMPS (sodium salt) physikalisch-chemischer Eigenschaften

storage temp.	Store at -20°C
Löslichkeit	DMF: 30 mg/ml; DMSO: 25 mg/ml; Ethanol: 0.5 mg/ml; PBS (pH 7.2): 10 mg/ml
Aggregatzustand	A crystalline solid

Rp-8-bromo-Cyclic AMPS (sodium salt) Chemische Eigenschaften,Einsatz,Produktion Methoden

Beschreibung
Rp-8-bromo-Cyclic AMPS (Rp-8-bromo-cAMPS) is a cell-permeable cAMP analog that combines an exocyclic sulfur substitution in the equatorial position of the cyclophosphate ring with a bromine substitution in the adenine base of cAMP.^1,2 It acts as an antagonist of cAMP-dependent protein kinases (PKAs) and is resistant to hydrolysis by cyclic nucleotide phosphodiesterases. Rp-8-bromo-cAMPS more effectively antagonizes cAMP-dependent activation of purified PKA type I from rabbit muscle than PKA type II from bovine heart.²
Verwenden
Rp-8-Br-cAMPS sodium is an analog of cAMP and an inhibitor of PKA. Rp-8-Br-cAMPS sodium occupies cAMP binding sites on PKA type I regulatory subunits, thereby preventing PKA dissociation and activation. Rp-8-Br-cAMPS sodium can be used in the study of tumors and retrovirus-induced immune deficiency. Rp-8-Br-cAMPS sodium also inhibits insulin secretion[1][2][3].

in vivo

Rp-8-Br-cAMPS (1 mg; intraperitoneal injection; 10 days) improves immune function in a mouse retrovirus infection model^[3].

Animal Model:	Murine leukemia retrovirus RadLV-Rs treated male C57BL/6 mice^[3]
Dosage:	1 mg
Administration:	Intraperitoneal injection (i.p.); 10 days
Result:	Had no significant effect on the extent of lymphadenopathy and splenomegaly which is typical of RadLV-Rs retroviral infection. Strongly increased responses to the anti-CD3 mAb. Improved T cell responses.

Einzelnachweise
1. Dostmann, W.R., Taylor, S.S., Genieser, H.G., et al. Probing the cyclic nucleotide binding sites of cAMP-dependent protein kinases I and II with analogs of adenosine 3',5'-cyclic phosphorothioates J. Biol. Chem. 265(18),10484-10491(1990).
2. Gjertsen, B.T., Mellgran, G., Otten, A., et al. Novel (Rp)-cAMPS analogs as tools for inhibition of cAMP-kinase in cell culture. Basal cAMP-kinase activity modulates interleukin-1β action J. Biol. Chem. 270(35),20599-20607(1995).