Mirin Struktur

Mirin

Bezeichnung:Mirin

CAS-Nr299953-00-7

Englisch Name:Mirin

CBNumberCB02501840

SummenformelC10H8N2O2S

Molgewicht220.25

MOL-Datei299953-00-7.mol

Mirin physikalisch-chemischer Eigenschaften

Schmelzpunkt	295-297 °C(Solv: ethanol (64-17-5))
Siedepunkt	441.6±55.0 °C(Predicted)
Dichte	1.49±0.1 g/cm3(Predicted)
storage temp.	2-8°C
Löslichkeit	DMSO: >10mg/mL
Aggregatzustand	Reddish orange powder
pka	9.15±0.30(Predicted)
Farbe	red to orange
Sensitive	Moisture Sensitive
Stabilität	Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 3 months.

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36
S-Sätze:	26
WGK Germany	3

Gefahreninformationscode (GHS)

Mirin Chemische Eigenschaften,Einsatz,Produktion Methoden

Beschreibung
Mirin is an inhibitor of the Mre11/Rad50/Nbs1 (MRN) complex, which functions as a DNA damage sensor and activates the DNA repair enzyme ataxia-telangiectasia mutated kinase (ATM). It inhibits phosphorylation of H2AX induced by DNA containing dsDNA breaks (DSBs), indicating decreased ATM activation, with an IC₅₀ value of 66 μM in X. laevis cell-free extracts. Mirin (100 μM) also inhibits the exonuclease activity of Mre11 in a cell-free assay. It induces cell cycle arrest at the G₂ phase in TOSA4 cells and inhibits homology-dependent repair in HEK293 cells in a concentration-dependent manner. Nanoparticle-encapsulated mirin (50 mg/kg) reduces tumor growth in an LA-N-5 mouse xenograft model.
Verwenden
Mirin has been used:
- as a small molecule inhibitor of meiotic recombination 11 (MRE11), in the pre-treatment of HCT116 wildtype cells before irradiation
- as Mre11 inhibitior in BSC-1 cells infected with simian virus 40
- in the pre-treatment of W12E cells for quantification of human papilloma virus (HPV) episome levels and to potentiate the effect of polyamide-25 (PA-25)
Verwenden
The Mre11-Rad50-Nbs1 (MRN) complex acts as a DNA damage sensor, maintains genome stability during DNA replication, and activates and recruits ataxia-telangiectasia mutated (ATM) to damaged DNA. The genes that encode the MRN proteins are all essential for cell viability, so functional studies of MRN cannot involve deletion of these genes. Mirin is an inhibitor of MRN, inhibiting MRN-dependent phosphorylation of histone H2AX (IC50 = 66 μM). Through its effects on MRN, mirin prevents activation of ATM. Inhibition of MRN centers on the ability of mirin to block the nuclease activity of Mre11, rather than altering DNA-binding or MRN complex formation. In cells, mirin induces G2 arrest, abolishes the radiation-induced G2/M checkpoint, and prevents homology-directed repair of DNA damage.[Cayman Chemical]
Biochem/physiol Actions
Mirin is an inhibitor of the Mre11-Rad50-Nbs1 complex. It disrupts nuclease activity of Mre11 and thus, the ability to repair DNA double strand breaks.
Einzelnachweise
1) Dupre et al. (2008), A forward chemical genetic screen reveals an inhibitor of the Mre11-Rad50-Nbs1 complex; Nat. Chem. Biol., 4 119

Mirin Upstream-Materialien And Downstream Produkte

Mirin Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

299953-00-7, Mirin Verwandte Suche:

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