CD68抗体(用于大鼠、小鼠)

兔多克隆抗体to CD68

Mouse, Rat

Synthetic peptide corresponding to Mouse CD68 aa 312-326 (internal sequence). Different from the related rat sequence by one amino acid.

CD68抗体(用于大鼠、小鼠)是可以特异性结合CD68的一类多克隆抗体，主要用于检测蛋白CD68的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。抗体是具有4条多肽链的对称结构，其中2条较长、相对分子量较大的相同的重链(H链);2条较短、相对分子量较小的相同的轻链(L链)。链间由二硫键和非共价键联结形成一个由4条多肽链构成的单体分子。轻链有κ和λ两种，重链有μ、δ、γ、ε和α五种。

WB, IHC-P, IHC-Fr

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)

CD68 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL ab125212 overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.