荧光染料

背景用途应用体外研究体内研究荧光染料试剂级价格

荧光染料

CAS号:210892-23-2
英文名:Cy5.5
中文名:荧光染料
CBNumber:CB8951968
分子式:C41H44N2O14S4
分子量:917.04
MOL File:210892-23-2.mol

荧光染料化学性质

形态 :Solid
颜色 :Purple to purplish red

荧光染料性质、用途与生产工艺

背景
Cy5.5是氰基染料的一种，氰基的定义是“合成染料，其通式为R2N[CH=CH]nCH=N+R2，分解为R2N+=CH[CH=CH]nNR2 (N是一个小数)，其中氮和部分共轭链通常构成杂环系统的一部分，如咪唑、吡啶、吡role、喹啉和噻唑。”氰基染料用于工业生物技术(标记、分析、生物医学成像)。
因为氰酸能产生更亮更稳定的荧光，所以可以很好地取代传统的染料，如荧光素和罗丹明。Cy3和Cy5是最常用的，通常用于两种颜色的检测。Cy3发出绿黄色荧光(约550 nm激发，约570 nm发射)，而Cy5在红色区域发出荧光(约650 nm激发，约670 nm发射)。
Cy5成为流行的红色荧光染料替代品是由于其高消光系数(在凝胶电泳可以肉眼发现1 nanomol)。Cy5.5是一种近红外(IR)荧光发射染料(激发/发射最大值678/694 nm)。
用途
荧光染料Cy5.5 (Sulfo-Cyanine5.5) 是用于标记生物分子的近红外荧光染料 (Ex=673 nm, Em=707 nm)，如肽，蛋白质和寡核苷酸。
应用
荧光染料主要能吸收可见光波和紫外光波，并能将紫外光转变为波长较长的可见光波而反射出来。例如，酸性曙红、荧光黄、红汞以及某些分散染料等。它们大多是含有苯环或杂环并带有共轭双键的化合物。
荧光染料具有闪光的鲜艳色彩，除用于纤维织物印染外，还可用作某些特种标志(如暗处符号)及军事追踪等。荧光染料中含有能与核酸、碳水化合物和肽共轭连接的基团，该共轭物一般情况下稳定性好，在紫外可见光的照射下，相比于其他的荧光物质或颜色标记物具有更优异的溶剂变色效应。它还可用于渗漏污水系统、水和工业的污染物、连接系统、测量发电厂排出的液体、洗手间的渗漏、非法的连接污水管监察，研究流量和绘图、分析腐败的系统。
体外研究

Cy5.5 contains a free amine group which can be conjugated with a variety of functionalities, including NHS esters, and epoxides. The excitation and emission maximum are 678 and 694 nm, respectively. Cy5.5 has been applied in various fluorescent based experiments. Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. Cy5.5 is cost-effective and its labeling chemistries are easy to perform, making it suitable for potential anti-cancer drug development.
体内研究

Cy5.5-labeled factor VIIa is developed for imagining cancer. Cy5.5 labeled with these targeting proteins specifically localize to the tumor xenografts for at least 14 days but unconjugated Cy5.5 does not localize to any xenografts or organs. This method of imaging anti-tissue factor in the tumor VECs may be useful in detecting primary tumors and metastases as well as monitoring in vivo therapeutic responses. pH/temperature sensitive magnetic nanogels conjugated with Cy5.5-labled lactoferrin (Cy5.5-Lf-MPNA nanogels) are developed as a promising contrast agent for preoperative MRI and intraoperative fluorescence imaging of glioma.