呋塞米钠

生物活性靶点体外研究体内研究呋塞米钠试剂级价格

呋塞米钠

CAS号:41733-55-5
英文名:sodium 5-(aminosulphonyl)-4-chloro-2-(furan-2-ylmethyl)anthranilate
中文名:呋塞米钠
CBNumber:CB7886067
分子式:C12H12ClN2NaO5S
分子量:354.74
MOL File:41733-55-5.mol

呋塞米钠化学性质

储存条件 :Store at -20°C
溶解度 :DMSO: ≥ 150 mg/mL (425.25 mM)

呋塞米钠性质、用途与生产工艺

生物活性 Furosemide sodium 是 Na+/K+/2Cl- (NKCC)，NKCC1 和 NKCC2 共转运蛋白的有效的和具有口服活性抑制剂。Furosemide sodium 也是 GABAA 受体拮抗剂，对含 α6 受体的选择性是含 α1 受体的 100 倍。Furosemide sodium 用作利尿试剂，用于充血性心力衰竭，高血压和水肿的研究。
靶点

IC50: NKCC1 and NKCC2IC50: GABA _A receptors
体外研究

Furosemide sodium (500 µM; 72-96 hours) significantly changes the proliferation rates in MKN45 cells (the poorly differentiated human gastric adenocarcinoma cell line). however, it has no effects on MKN28 cells (the moderately differentiated human gastric adenocarcinoma cell line). The growth rate of MKN45 cells is larger than that of MKN28 cells.Furosemide sodium (10 µM, 30 µM, 100 µM; 45 min exposure)significantly decreases cation channel activity and [Ca(2+)](i) in human erythrocytes drawn from healthy individuals. Tert-butylhydroperoxide similarly enhances the non-selective cation channels activity, increases [Ca(2+)](i) and triggered cell membrane scrambling, however, the effects is significantly blunted by Furosemide sodium again.
体内研究

Furosemide sodium (intraperitoneal injection; 100 mg/kg; single dose) is injected after kanamycin (KM) (1000 mg/kg) to creat a deaf mouse model in C57BL/6 mouse. After injection, hearing loss and cochlear hair cell damage are evaluated on day 1, day 2 and day 3, respectively. The hearing is markedly deteriorated even from the next day (Day-1 group), OHCs (outer hair cell) morphology of apical, middle and basal turns are disorganized in mice on day3.