生物活性 体外研究 荧光素 试剂级价格
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荧光素

荧光素,2321-07-5,结构式
荧光素
  • CAS号:2321-07-5
  • 英文名:Fluorescein
  • 中文名:荧光素
  • CBNumber:CB7753558
  • 分子式:C20H12O5
  • 分子量:332.31
  • MOL File:2321-07-5.mol
荧光素化学性质
  • 熔点 :320 °C(lit.)
  • 沸点 :429.44°C (rough estimate)
  • 密度 :1.2739 (rough estimate)
  • 折射率 :1.5000 (estimate)
  • 储存条件 :room temp
  • 溶解度 :Solubility Insoluble in water, ether, benzene, chloroform; soluble in ethanol, methanol, acetone, Iethyl acetate, N,N-dimethylformamide
  • 色指数 :45350
  • 形态 :Powder
  • 酸度系数(pKa) :2.2, 4.4, 6.7(at 25℃)
  • 颜色 :Red to orange
  • 酸碱指示剂变色ph值范围 :Pink uorescence (4.0) to green uorescence (6.0)
  • 水溶解性 :insoluble
  • 最大波长(λmax) :493.5nm, 496nm, 460nm, 515nm
  • Merck :14,4159
  • BRN :94324
  • 稳定性 :Stable. Combustible. Incompatible with strong oxidizing agents.
  • 主要应用 :Organic light-emitting diode, nanoparticles, liquid crystal display, oil products, lip make-up, nucleic acid synthesis, amplification, sequencing and cloning, diagnosis of diabetic retinopathy, detecting yeast, multidrug resistance, proteins, antiHCV antibodies, target genes, enzymatic activity, nerve agent, nucleic acid sequences, imaging lung cancer, prostate cancer
  • 生物领域应用 :Detecting chromosomal aberration; diagnosing corneal diseases; marking/detecting insects; as antitumor agent; as antihistaminic agent; use in agriculture and plant cultivation; use in cosmetics; use in ophthalmology; as a substrate for measuring α-amylases activity, elastases activity, esterases activity, β-glucuronidases activity, horseradish peroxidases activity, kinases activity, monoamine oxidases (MAOs) activity, phosphatases activity, phospholipases activity, proteases/proteinases activity, telomerases activity activity
  • LogP :2.980 (est)
  • CAS 数据库 :2321-07-5(CAS DataBase Reference)
  • EPA化学物质信息 :Fluorescein (2321-07-5)
安全信息

荧光素 MSDS


2-(6-Hydroxy-3-oxo-(3H)-xanthen-9-yl)benzoic acid

荧光素性质、用途与生产工艺

  • 生物活性 Fluorescein (Resorcinol phthalein) 是一种合成的有机光敏染料化合物。Fluorescein 是荧光示踪剂,可以应用于医学和生物学以及肿瘤感染的组织。
  • 体外研究

    Fluorescein is nearly inactive as a photodynamic sensitizer. Fluorescein consequently is almost useless as a photodynamic sensitizer, but it is of great value in fluorescence immunoassays.
    The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent.

  • 化学性质 

    荧光素外观呈橙黄或浅橙红色结晶粉末。熔点314-316℃(分解)。溶于热乙醇、冰醋酸、碱溶液,呈绿色萤光,不溶于水、苯、氯仿和乙醚。

  • 用途 

    荧光素是汞溴红的中间体,可用作萤光光度分析试剂,测定氯、溴、碘、硫离子;氧化-还原指示试剂;生物染色剂。

  • 用途  荧光光度分析硫离子,试验溴,滴定氯、溴和碘。吸附指示剂,氧化还原指示剂。
  • 用途  吸附指示剂;氧化还原指示剂;荧光光度分析硫离子;滴定氯、溴和碘
  • 生产方法 

    荧光素可用邻苯二甲酸酐与间苯二酚在硫酸参与下反应制得。

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荧光素 试剂级价格
  • 更新日期:2024/01/25
  • 产品编号:HY-D0251
  • 产品名称:荧光素 Fluorescein
  • CAS编号:2321-07-5
  • 包装:250mg
  • 价格:500元
  • 更新日期:2024/01/25
  • 产品编号:HY-D0251
  • 产品名称:荧光素 Fluorescein
  • CAS编号:2321-07-5
  • 包装:10mM * 1mLin DMSO
  • 价格:550元
荧光素生产厂家
  • 公司名称:Portail Substances Chimiques
  • 联系电话:--
  • 电子邮件:webmestre-substances@ineris.fr
  • 国家:法国
  • 产品数:6027
  • 优势度:58
  • 公司名称:EDQM
  • 联系电话:--
  • 电子邮件:orders@edqm.eu
  • 国家:法国
  • 产品数:1914
  • 优势度:81
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