黄羽扇豆魏特酮

生物活性靶点体外研究黄羽扇豆魏特酮试剂级价格

黄羽扇豆魏特酮

CAS号:104691-86-3
英文名:8-PRENYLGENISTEIN
中文名:黄羽扇豆魏特酮
CBNumber:CB7246036
分子式:C20H18O5
分子量:338.35
MOL File:Mol file

黄羽扇豆魏特酮化学性质

熔点 :133-135℃
沸点 :583.5±50.0 °C(Predicted)
密度 :1.351±0.06 g/cm3(Predicted)
储存条件 :4°C, protect from light
溶解度 :DMSO : 100 mg/mL (295.55 mM; Need ultrasonic)
形态 :Powder
酸度系数(pKa) :6.62±0.20(Predicted)
颜色 :Off-white to light yellow

黄羽扇豆魏特酮性质、用途与生产工艺

生物活性 Lupiwighteone 是一种广泛存在于野生植物中的异黄酮，具有抗氧化、抗菌和抗癌作用。Lupiwighteone 通过抑制 PI3K/Akt/mTOR 通路，诱导人乳腺癌细胞 caspase 依赖性和非依赖性凋亡 (apoptosis)。
靶点

apoptosis

体外研究

Lupiwighteone (2-100 μM; 72 hours) shows cytotoxicity towards various cell lines, especially on DU-145 cells and SGC-7901 cells with IC ₅₀ s of 23.7 μM and 21 μM, respectively.
Lupiwighteone (20-60 μM; 48 hours) induces (cell cycle arrest in DU-145 cells.
Lupiwighteone (20-60 μM; 48 hours) induces cells apoptosis.
Lupiwighteone (20-60 μM; 48 hours) decreases the cell cycle-related protein expressions in a dose-dependent manner in DU-145 cells.
Lupiwighteone (20-60 μM; 48 hours) induces a dose-dependent increase in ROS production.
Lupiwighteone up-regulates of cytochrome c and caspase-3, and subsequent cleavage of PARP-1 and down-regulates of the p-Akt/Akt ratio and VEGF expression, suggests the activation of mitochondria-based intrinsic apoptosis in DU-145 cells.

Cell Viability Assay

Cell Line:	DU-145 cells, C4-2 cells, SGC-7901 cells, Hela cells, HUVEC, MCF-7 cells, CNE cells, Vero
Concentration:	2 μM, 5 μM, 25 μM, 50 μM, 100 μM
Incubation Time:	72 hours
Result:	Had inhibition on the survival of many cancer cell lines and HUVEC.

Cell Cycle Analysis

Cell Line:	DU-145 cells
Concentration:	20 μM, 40 μM, 60 μM
Incubation Time:	48 hours
Result:	Induced cell cycle arrest.

Apoptosis Analysis

Cell Line:	DU-145 cells
Concentration:	20 μM, 40 μM, 60 μM
Incubation Time:	48 hours
Result:	Induced apoptosis.

Western Blot Analysis

Cell Line:	DU-145 cells
Concentration:	20 μM, 40 μM, 60 μM
Incubation Time:	48 hours
Result:	Decreased CDK1, 2, 4, 6, cyclinD1, and cyclinB1 protein expression in a dose-dependent manner.