软木肟酸

作用用途生物活性靶点体外研究体内研究软木肟酸试剂级价格

软木肟酸

CAS号:38937-66-5
英文名:Suberohydroxamic acid
中文名:软木肟酸
CBNumber:CB6673028
分子式:C8H16N2O4
分子量:204.22
MOL File:38937-66-5.mol

软木肟酸化学性质

熔点 :153-155 °C(lit.)
密度 :1.219±0.06 g/cm3(Predicted)
储存条件 :−20°C
溶解度 :H₂O: soluble
酸度系数(pKa) :9.18±0.20(Predicted)
形态 :lyophilized powder
颜色 :White to Light Beige
稳定性 :Hygroscopic

安全信息

安全说明 :22-24/25
WGK Germany :3
海关编码 :2928009090

软木肟酸性质、用途与生产工艺

作用 Suberoylbis-hydroxamic酸(软木肟酸)作为第二代异羟肟酸类组蛋白去乙酰化酶抑制剂，能够抑制组蛋白去乙酰化酶中HDAC1和HDAC3的活性，小剂量、低浓度时可诱导肿瘤分化，并选择性地抑制肿瘤生长而对正常细胞无明显不良反应。通过对乳腺癌的研究发现软木肟酸呈时间、剂量依赖性抑制乳腺癌细胞增殖，使其停滞于G0～G1期。ChemicalBook
用途软木肟酸是一种组蛋白去乙酰化酶抑制剂，它可能具备激活Notch1信号通路的功能。应用Western实验检验Notch1信号通路被激活后所表达的活性蛋白NICD，来判断软木肟酸对Notch1信号通路的作用。
生物活性 Suberohydroxamic acid (suberic bishydroxamic acid)是竞争性HDAC抑制剂，对HDAC1和HDAC3的IC50分别为0.25 μM和0.3 μM。
靶点
Target Value
HDAC1
(Cell-free assay) 0.25 μM
HDAC3
(Cell-free assay) 0.3 μM

体外研究

Suberoyl bis-hydroxamic acid (10, 20 or 50 μM; 24 hours) combination with TRAIL improves apoptosis extent, and when TRAIL is combined with 20 μM SBHA (itself causing only 10–15% apoptosis), resulting in 45–50% cell death.Suberoyl bis-hydroxamic acid (20-50 μM; 10-20 hours) alone has little effect on the expression of the proteins Bcl-xL, Mcl-1, and has albeit mildeffect on Bax. When it combines with TRAIL,which increases the ratio of relative protein expression of Bcl-xL and Bax in early periods, while the change in the ratio of Mcl-1 and Bax increases later in MM-BI and Ist-Mes2 cells.Suberoyl bis-hydroxamic acid (30 μM; 6 hours) causes accumulation of acetylated histone H4 in MEL cells.

Apoptosis Analysis

Cell Line:	MM-BI and Ist-Mes2 cells
Concentration:	10 μM, 20 μM or 50 μM
Incubation Time:	24 hours
Result:	Showed a cooperative effect in cell apoptosis.

体内研究

Suberoyl bis-hydroxamic acid (intraperitoneal injection; 200 mg/kg; every 2 days; 12 days) reveals a marked increase in the active form of Notch1 (NICD) with a concomitant decrease in ASCL1. It reduces the MTC tumor growth.

Animal Model:	Nude mice injected with human MTC cells
Dosage:	200 mg/kg
Administration:	Intraperitoneal injection; every 2 days; 12 days
Result:	Resulted in an average 55% inhibition of tumor growth in SBHA treatment group.