ANTI-NESTIN抗体

兔单克隆抗体 to Nestin

Rabbit

适用于: ICC/IF, Flow Cyt, WB, IHC-P

与反应: Human
预测可用于: Dog

Synthetic peptide within Human Nestin aa 1600 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P48681

Western blot - Anti-Nestin antibody [SP103] (ab105389)

All lanes : Anti-Nestin antibody [SP103] (ab105389) at 1/100 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : NES knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 177 kDa
Lanes 1 - 2: Merged signal (red and green). Green - ab105389 observed at 300 kDa. Red - loading control, ab18058, observed at 117 kDa.
ab105389 was shown to specifically react with NES in wild-type HAP1 cells as signal was lost in NES knockout cells. Wild-type and NES knockout samples were subjected to SDS-PAGE. Ab105389 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

ANTI-NESTIN抗体是一类可以特异性结合ANTI-NESTIN的多克隆抗体，主要用于检测ANTI-NESTIN的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。

双抗体夹心法测定标本中ANTI-NESTIN水平。用纯化的ANTI-NESTIN抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入ANTI-NESTIN，再与HRP标记的ANTI-NESTIN抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的ANTI-NESTIN呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中ANTI-NESTIN浓度。

Nestin是一种属于第Ⅵ类中间丝的细胞骨架蛋白,表达于多种组织的干细胞或前体细胞中,被广泛用作干细胞的标志性蛋白。除维持细胞的正常形态,nestin还具有抗凋亡作用。抑制细胞中nestin的表达,可以增强过氧化氢(H202)刺激诱导的神经前体细胞和血管平滑肌细胞的凋亡,而将野生型的nestin(nest-1893)转入到细胞内可起到保护作用,显著减少H202诱导的细胞凋亡。