FLAG肽

生物活性体外研究 FLAG肽试剂级价格

FLAG肽

CAS号:98849-88-8
英文名:FLAG peptide
中文名:FLAG肽
CBNumber:CB31395616
分子式:C41H60N10O20
分子量:1012.97
MOL File:98849-88-8.mol

FLAG肽化学性质

密度 :1.470±0.06 g/cm3 (20 ºC 760 Torr)
储存条件 :Keep in dark place,Sealed in dry,Store in freezer, under -20°C
溶解度 :≥50.65 mg/mL in DMSO; ≥210.6 mg/mL in H2O; ≥34.03 mg/mL in EtOH
形态 :Lyophilized powder
颜色 :White to off-white
序列 :H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH
InChIKey :XZWYTXMRWQJBGX-OVIGALBNNA-N

FLAG肽性质、用途与生产工艺

生物活性 FLAG peptide是一种八个氨基酸组成的多肽 (Asp-Ty-rLys-Asp-Asp-Asp-Asp-Lys)，具有肠激酶切割位点。FLAG peptide用于抗体介导的重组蛋白的识别和纯化。
体外研究

Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca ²⁺ . Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca ²⁺ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions.
多肽 FLAG Peptide（Flag-tag Peptide）是编码8个氨基酸的亲水性多肽（DYKDDDDK），含有肠激酶酶切位点，可用于免疫亲和层析。Flag多肽可在非变性条件下洗脱蛋白，有多种抗体可识别Flag多肽，如M1、M2和M5。
参考质量标准外观:白色粉末
纯度(HPLC) ≥98.0%
醋酸根含量≤12.0%
水分含量≤8.0%
肽含量≥80.0%
内毒素≤50EU/mg
氨基酸组成分析≤±10%