香橙素

生物活性靶点体外研究香橙素试剂级价格

香橙素

CAS号:480-20-6
英文名:DIHYDROKAEMPFEROL
中文名:香橙素
CBNumber:CB1772858
分子式:C15H12O6
分子量:288.25
MOL File:480-20-6.mol

香橙素化学性质

熔点 :237～241℃
沸点 :139-140 °C
密度 :1.599±0.06 g/cm3(Predicted)
储存条件 :Sealed in dry,2-8°C
溶解度 :DMSO:2.88(Max Conc. mg/mL);10.0(Max Conc. mM)
酸度系数(pKa) :7.42±0.60(Predicted)
InChIKey :PADQINQHPQKXNL-LSDHHAIUSA-N
LogP :2.860 (est)
EPA化学物质信息 :4H-1-Benzopyran-4-one, 2,3-dihydro-3,5,7-trihydroxy-2-(4-hydroxyphenyl)-, (2R,3R)- (480-20-6)

安全信息

危险品标志 :N,Xn
危险类别码 :50-22
安全说明 :61
危险品运输编号 :UN 3077 9 / PGIII
WGK Germany :3

香橙素性质、用途与生产工艺

生物活性 Dihydrokaempferol 是从紫荆花 Bauhinia championii (Benth) 中分离得到的。 Dihydrokaempferol 诱导 apoptosis 并抑制 Bcl-2 和 Bcl-xL 的表达。Dihydrokaempferol 可以作为抗关节炎新药的良好候选。
靶点

Apoptosis; Bcl-2; Bcl-xL

体外研究

Dihydrokaempferol (0.3-300 μM; 48 hours) has no significant effect on normal synoviocytes, but dependently decreases the viability of RA-FLSs. Dihydrokaempferol (3-30 μM; 48 hours) increases the percentage of apoptotic cells (including early and late apoptotic cells; ~3.8% and ~9.6%, respectively). Dihydrokaempferol (3-30 μM; 48 hours) promotes Bax and Bad expression and inhibits Bcl-2 and Bcl-xL expression, increases the cleaved fragments of caspase-3, caspase-9 and cleaved PARP.

Cell Proliferation Assay

Cell Line:	Normal synoviocyte cells; RA-FLS cells
Concentration:	0.3 μM, 3 μM, 30 μM, 300 μM
Incubation Time:	48 hours
Result:	Decreased the proliferation of RA-FLSs.

Apoptosis Analysis

Cell Line:	RA-FLS cells
Concentration:	3 μM, 30 μM
Incubation Time:	48 hours
Result:	Induced apoptosis in RA-FLSs.

Western Blot Analysis

Cell Line:	RA-FLS cells
Concentration:	3 μM, 30 μM
Incubation Time:	48 hours
Result:	Promoted Bax and Bad expression, increased the cleaved fragments of caspase-3, caspase-9 and cleaved PARP and inhibited Bcl-2 and Bcl-xL expression.