异硫氰酸荧光素酯

简介用途生物活性体外研究异硫氰酸荧光素酯试剂级价格

异硫氰酸荧光素酯

CAS号:3326-32-7
英文名:Fluorescein isothiocyanate isomer I
中文名:异硫氰酸荧光素酯
CBNumber:CB0173162
分子式:C21H11NO5S
分子量:389.38
MOL File:3326-32-7.mol

异硫氰酸荧光素酯化学性质

熔点 :>360 °C(lit.)
沸点 :708.6±60.0 °C(Predicted)
密度 :1.3423 (rough estimate)
折射率 :1.5060 (estimate)
储存条件 :2-8°C
溶解度 :Slightly soluble in DMSO, ethanol, methanol DMF and acetone
形态 :powder
酸度系数(pKa) :2.2, 4.4, 6.7(at 25℃)
颜色 :White
酸碱指示剂变色ph值范围 :Weak green uorescence (6.0) to strong green uorescence (7.2)
水溶解性 :practically insoluble
敏感性 :Moisture Sensitive
最大波长(λmax) :490nm
BRN :1407295
稳定性 :Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids.
主要应用 :Magnetic nanowires, immunoassays, analyzing proteins, identifying chromosomes, Salmonella mosomes, diagnosis of cancer, kidney diseases, detecting pathogens, genes, Salmonella cells, toxoplasmosis, enzyme-mediated nucleic acid cleavage, surface molecules on colorectal cancers, treating cancer, chronic lymphocytic leukemia
生物领域应用 :Analyzing sugar chain; detecting iodide/iodate or total iodine; detecting/imaging thiols; detecting/quantifying biotin derivatives;16 diagnosing classical Hodgkin lymphoma (CHL) in lymph nodes; cellulose nanocrystals for bioimaging applications; treating neuroinflammation; treating/diagnosing cancer; PEO-PCL-PEO triblock copolymers for topical delivery; polymersomes in biomedical or cosmetic applications;apoptosis assay; as a substrate for measuring heparanase activity, human immunodeficiency virus (HIV-1) proteinase activity, kinase activity, phosphatase activity, β-lactamase activity, transglutaminase (TG2) activity; implantable drug-delivery devicess
InChIKey :MHMNJMPURVTYEJ-UHFFFAOYSA-N
CAS 数据库 :3326-32-7(CAS DataBase Reference)
EPA化学物质信息 :Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato- (3326-32-7)

安全信息

危险品标志 :Xn,Xi
危险类别码 :42/43-36/37/38-22
安全说明 :22-45-36/37/39-26-36/37-36
WGK Germany :3
F :10
TSCA :Yes
海关编码 :29329990

异硫氰酸荧光素酯性质、用途与生产工艺

简介
异硫氰酸荧光素酯是黄色粉末，有吸湿性。其能与各种抗体蛋白结合，结合后的抗体不丧失与一定抗原结合的特异性，并在碱性溶液中仍有强烈绿色荧光，加酸后析出沉淀，荧光消失，微溶于丙酮、乙醚和石油醚。
用途异硫氰酸荧光素酯能和各种抗体蛋白结合，结合后的抗体不丧失与一定抗原结合的特异性，并在碱性溶液中具有强烈的黄绿色荧光。通过在荧光显微镜下观察或流式细胞仪分析可对相应抗原进行定性、定位或定量的检测。用于医学，农学和畜牧等方面，可对由细菌病毒和寄生虫等所致疾病进行快速诊断。
生物活性 FITC (Fluorescein 5-isothiocyanate) 是一种用于胺标记的荧光探针。FITC 是对 pH 和 Cu2+ 敏感的荧光染料。
体外研究

Annexin V-FITC/PI is employed to detect chondrocyte apoptosis. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites.
用途蛋白质荧光标记试剂。用于荧光抗体技术快速鉴定病原体。染料和代谢物。
用途生化研究。荧光抗体示踪。病毒和寄生虫等所致疾病的快速诊断。