76421-73-3
基本信息
单氯二胺 MBCL
MBCL [单氯二胺]
MBCL
CHLOROBIMANE
THIOLYTE(R) MC
MONOCHLOROBIMANE
THIOLYTE(R) MC REAGENT
MBCl [MonochlorobiMane]
THIOLYTE(R) MONOCHLOROBIMANE REAGENT
mBCl [Monochlorobimane] *CAS 76421-73-3*
THIOLYTE(R) MONOCHLOROBIMANE REAGENT, FLUORESCENT LABELING COMPOUND
物理化学性质
| 熔点 | 135-136 °C(lit.) |
| 沸点 | 314.9±44.0 °C(Predicted) |
| 密度 | 1.40±0.1 g/cm3(Predicted) |
| 储存条件 | Keep in dark place,Inert atmosphere,2-8°C |
| 溶解度 | DMF: soluble |
| 酸度系数(pKa) | -3.22±0.70(Predicted) |
| 形态 | Solid |
| 颜色 | Light yellow to yellow |
| BRN | 4440901 |
安全数据
| 危险性符号(GHS) |  GHS07 |
| 警示词 | 警告 |
| 危险性描述 | H315-H319-H335 |
| 防范说明 | P261-P264-P271-P280-P302+P352-P305+P351+P338 |
| 危险品标志 | Xi |
| 危险类别码 | 36/37/38 |
| 安全说明 | 26-36 |
| WGK Germany | 3 |
| F | 8 |
常见问题列表
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of control and experimental wells:
The experiment should consist of parallel negative, positive and experimental wells respectively.
Experimental wells: add 200 μL of cell culture media containing your GSH effector of interest at desired concentration (e.g. 200 µM H
2
O
2
)
2. Incubate the plate overnight at 37 °C, 5 % CO
2
. The incubation time varies depended on your normal protocol
3. Monochlorobimane (mBCl) is added to the cells at a final concentration of
20-100 μM
from a working solution of 1 mM. The stock solution of mBCl (50 mM) was prepared in dimethyl sulphoxide (DMSO) and stored at -20°C; the working solution was prepared before use by diluting the stock solution in 0.1 M PBS buffer (pH 7.0). In the assay the final concentration of DMSO was below 0.2% (v/v)
4. The cell suspensions were incubated with mBCl in the dark at 25°C for ~2 h
5. Remove the dye and treatment by centrifugation at 700 X g for 5 min. Add 200 µL of the Assay Buffer to each well and continue to the preferred method of detection
6. Detection of intracellular GSH:
Fluorescence plate reader: For adherent cells; read fluorescence directly off the culturing plate. If working with suspension cells; aliquot 200 µL of the culture suspension into the white opaque plate and record fluorescence at E
X
/E
M
= 380/465 ±20 nm respectively (
blue
).