9013-31-4

Apoferritin from equine spleen has been used:

• to determine its partition coefficients in phase systems
• in NaCl for iron loading of cultured cells
• in in situ liquid scanning transmission electron microscope for imaging the interface of biology and nanotechnology

Apoferritin is a gel filtration molecular weight marker that can be used in gel filtration chromatography and protein chromatography. Apoferritin has the ability to demetallate hemin and to metallate protoporphyrin IX.

Apoferritin is a gel filtration molecular weight marker that can be used in gel filtration chromatography and protein chromatography. Apoferritin has the ability to demetallate hemin and to metallate protoporphyrin. Apoferritin is used as a novel nanocarrier to load methylene blue (MB) and demonstrate its potential applications for photodynamic therapy of cancer.

This protein shell of ferritin lacking iron is widely used for the calibration of gel filtration columns and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. It is very abundant in beta-cells of the pancreas where it acts as an anti-oxidant. When added to cultured endothelial cells apoferritin is taken up in a dose-responsive manner and appears to protect the cells from oxidant-mediated cytolysis.